PMID- 18299423
OWN - NLM
STAT- MEDLINE
DCOM- 20080514
LR  - 20131121
IS  - 1741-7899 (Electronic)
IS  - 1470-1626 (Linking)
VI  - 135
IP  - 3
DP  - 2008 Mar
TI  - Spermatotoxic effect of aflatoxin B1 in rat: extrusion of outer dense fibres and 
      associated axonemal microtubule doublets of sperm flagellum.
PG  - 303-10
LID - 10.1530/REP-07-0367 [doi]
AB  - Male Wistar rats were treated with aflatoxin B1 (AFB1). Live as well as 
      methanol-fixed cauda epididymal spermatozoa were stained with acridine orange 
      (AO) and ethidium bromide (EB) and observed under a fluorescence microscope. 
      Giemsa-stained smears were observed in a bright field microscope. Unstained 
      smears were observed with phase contrast illumination. The axoneme of more than 
      10% of the spermatozoa of treated rats had the outer dense fibres (ODFs), in 
      varying numbers, and the associated axonemal microtubule doublets of the 
      flagellum extruded either at midpiece-principal piece junction or connecting 
      piece. This could be perceived in all light microscopic preparations, but AO-EB 
      staining offered an advantage of the assessment of the viability as well. TEM 
      observation of sections of the testis and cauda epididymidis also revealed ODF 
      extrusion, as seen in the transverse sections of sperm flagella missing one or 
      more ODFs and the associated axonemal microtubule doublets. In a few such 
      sections, the extruded elements were seen in the cytoplasm, outside the 
      mitochondrial sheath or peripheral sheath. Marginal to severe mitochondrial 
      pathologies were observed in the spermatozoa and elongated spermatids, suggesting 
      a link between AFB1-induced sperm mitochondrial pathology and extrusion of ODFs. 
      However, the possibility that AFB1 treatment would disrupt the cytoskeletal 
      proteins of the flagellum, resulting in the extrusion of ODFs, cannot be 
      excluded. This sperm abnormality is reported for the first time as produced by a 
      dietary toxin. Dietary aflatoxins, therefore, could also be contributory factors 
      for the deterioration of the reproductive health of men.
FAU - Faisal, K
AU  - Faisal K
AD  - Department of Animal Science, School of Life Sciences, Bharathidasan University, 
      Tiruchirappalli 620 024, India.
FAU - Periasamy, V S
AU  - Periasamy VS
FAU - Sahabudeen, S
AU  - Sahabudeen S
FAU - Radha, A
AU  - Radha A
FAU - Anandhi, R
AU  - Anandhi R
FAU - Akbarsha, M A
AU  - Akbarsha MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Reproduction
JT  - Reproduction (Cambridge, England)
JID - 100966036
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Poisons)
RN  - 9N2N2Y55MH (Aflatoxin B1)
SB  - IM
MH  - Aflatoxin B1/*pharmacology
MH  - Animals
MH  - Axoneme/drug effects/ultrastructure
MH  - Cytoskeletal Proteins/analysis
MH  - Epididymis
MH  - *Food Contamination
MH  - Injections, Intramuscular
MH  - Male
MH  - Microscopy, Electron, Transmission
MH  - Microscopy, Fluorescence
MH  - Microscopy, Phase-Contrast
MH  - Microtubules/drug effects/ultrastructure
MH  - Poisons/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Sperm Tail/drug effects/ultrastructure
MH  - Spermatozoa/*drug effects/ultrastructure
MH  - Staining and Labeling
MH  - Testis
EDAT- 2008/02/27 09:00
MHDA- 2008/05/15 09:00
CRDT- 2008/02/27 09:00
PHST- 2008/02/27 09:00 [pubmed]
PHST- 2008/05/15 09:00 [medline]
PHST- 2008/02/27 09:00 [entrez]
AID - 135/3/303 [pii]
AID - 10.1530/REP-07-0367 [doi]
PST - ppublish
SO  - Reproduction. 2008 Mar;135(3):303-10. doi: 10.1530/REP-07-0367.