PMID- 18301245
OWN - NLM
STAT- MEDLINE
DCOM- 20090514
LR  - 20161020
IS  - 1533-4058 (Electronic)
IS  - 1533-4058 (Linking)
VI  - 16
IP  - 3
DP  - 2008 May
TI  - Diagnostic accuracy of FISH and RT-PCR in 50 routinely processed synovial 
      sarcomas.
PG  - 246-50
LID - 10.1097/PAI.0b013e31815349f5 [doi]
AB  - BACKGROUND: Molecular detection of SYT-SSX fusion genes is the most reliable tool 
      for diagnosing synovial sarcoma (SS). The objective of this study was to 
      investigate the accuracy of reverse transcription-polymerase chain reaction 
      (RT-PCR) and a commercially available fluorescence in situ hybridization (FISH) 
      technique for formalin-fixed and paraffin-embedded tumor tissue. PATIENTS AND 
      METHODS: Fifty tumors with typical SS histology and 12 histologic mimics of SS 
      were included. RT-PCR for SYT-SSX1/SSX2 gene fusions and FISH analysis for SYT 
      gene breaks were performed on these 62 formalin-fixed and paraffin-embedded 
      tumors. RESULTS: All 50 SS were positive by either RT-PCR or FISH. Forty-seven SS 
      (94%) were true positive by RT-PCR and 41 SS (82%) were true positive by FISH. 
      FISH and RT-PCR results were interpretable and concordant in 38 cases (76%). Two 
      cases were not interpretable by RT-PCR and 6 cases were not interpretable by 
      FISH. One SS was false-negative with RT-PCR and 3 SS were false-negative with 
      FISH. RT-PCR and FISH had a sensitivity of 94% and 82%, a specificity and 
      positive predictive value of 100% and 100% and a negative predictive value of 80% 
      and 75%, respectively. CONCLUSIONS: RT-PCR had a higher sensitivity than FISH. 
      One of both methods was always positive, whereas both methods were concordant in 
      76% of cases. From an economic point of view, we advocate to use FISH as a method 
      of first choice, because it allows microscopic control of a true positive result 
      (unpaired fluorescent signals in a break apart assay). Using this approach, 80% 
      of SS can be diagnosed by FISH only and 20% would need to be confirmed by RT-PCR.
FAU - Ten Heuvel, Suzan E
AU  - Ten Heuvel SE
AD  - Department of Surgical Oncology, University Medical Center Groningen, University 
      of Groningen, The Netherlands.
FAU - Hoekstra, Harald J
AU  - Hoekstra HJ
FAU - Suurmeijer, Albert J H
AU  - Suurmeijer AJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Appl Immunohistochem Mol Morphol
JT  - Applied immunohistochemistry & molecular morphology : AIMM
JID - 100888796
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (SYT-SSX fusion protein)
RN  - 1HG84L3525 (Formaldehyde)
SB  - IM
MH  - Biomarkers, Tumor
MH  - Formaldehyde
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/economics/*methods
MH  - Oncogene Proteins, Fusion
MH  - Paraffin Embedding
MH  - Predictive Value of Tests
MH  - Reverse Transcriptase Polymerase Chain Reaction/economics/*methods
MH  - Sarcoma, Synovial/*diagnosis/metabolism/pathology
MH  - Sensitivity and Specificity
MH  - Soft Tissue Neoplasms/*diagnosis/metabolism/pathology
MH  - Tissue Fixation
EDAT- 2008/02/28 09:00
MHDA- 2009/05/15 09:00
CRDT- 2008/02/28 09:00
PHST- 2008/02/28 09:00 [pubmed]
PHST- 2009/05/15 09:00 [medline]
PHST- 2008/02/28 09:00 [entrez]
AID - 10.1097/PAI.0b013e31815349f5 [doi]
PST - ppublish
SO  - Appl Immunohistochem Mol Morphol. 2008 May;16(3):246-50. doi: 
      10.1097/PAI.0b013e31815349f5.