PMID- 18302964
OWN - NLM
STAT- MEDLINE
DCOM- 20080609
LR  - 20080314
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 82
IP  - 13-14
DP  - 2008 Mar 26
TI  - Molecular profiling of genes in squamous cell lung carcinoma in Asian Indians.
PG  - 772-9
LID - 10.1016/j.lfs.2008.01.009 [doi]
AB  - Early metastasis and a poor five-year survival make lung cancer the leading cause 
      of cancer related deaths worldwide. The clinical profile of lung cancer patients 
      in India differs from the West as they present earlier, with squamous cell 
      carcinoma being the commonest histological type. We compared gene expression 
      profiles in primary lung squamous cell carcinoma (LSCC) and matched normal lung 
      tissues in Asian Indians. Using suppression subtractive hybridization, two 
      subtracted cDNA libraries containing differentially expressed genes in the tumors 
      were constructed. Differential expression was confirmed by reverse Northern blot 
      analysis. DNA of confirmed clones was sequenced and subjected to GenBank Blast 
      searches. RNA expression levels were then analyzed by Northern blotting and 
      validated by semiquantitative RT-PCR (in 10 cases of NSCLC). Seventeen 
      differentially expressed gene cDNA fragments of LSCC were analyzed. The 
      differentially expressed genes included those associated with cellular 
      metabolism, cell-cycle, -structure, -adhesion, transcription, proliferation, 
      apoptosis and signal transduction. The study provided first evidence that 
      KIAA0767, a Death Inducing Protein, a novel p53 independent target of E2F1, and 
      Geminin, an inhibitor of DNA replication are differentially expressed in LSCC. 
      Identification of the differentially expressed genes in lung cancer in this study 
      may serve as better molecular markers for early diagnosis and identifying novel 
      intervention sites for anticancer therapy.
FAU - Sen, Sudip
AU  - Sen S
AD  - Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, 
      110029, India.
FAU - Ateeq, Bushra
AU  - Ateeq B
FAU - Sharma, Himani
AU  - Sharma H
FAU - Datta, Palika
AU  - Datta P
FAU - Gupta, Siddhartha Datta
AU  - Gupta SD
FAU - Bal, Sabyasachi
AU  - Bal S
FAU - Kumar, Arvind
AU  - Kumar A
FAU - Singh, Neeta
AU  - Singh N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080130
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (DNA, Complementary)
SB  - IM
MH  - Blotting, Northern
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - DNA, Complementary/genetics
MH  - *Gene Expression
MH  - *Gene Expression Profiling
MH  - Humans
MH  - India
MH  - Lung/metabolism/pathology
MH  - Lung Neoplasms/*genetics/pathology
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2008/02/28 09:00
MHDA- 2008/06/10 09:00
CRDT- 2008/02/28 09:00
PHST- 2007/08/06 00:00 [received]
PHST- 2007/11/20 00:00 [revised]
PHST- 2008/01/18 00:00 [accepted]
PHST- 2008/02/28 09:00 [pubmed]
PHST- 2008/06/10 09:00 [medline]
PHST- 2008/02/28 09:00 [entrez]
AID - S0024-3205(08)00039-8 [pii]
AID - 10.1016/j.lfs.2008.01.009 [doi]
PST - ppublish
SO  - Life Sci. 2008 Mar 26;82(13-14):772-9. doi: 10.1016/j.lfs.2008.01.009. Epub 2008 
      Jan 30.