PMID- 18303564
OWN - NLM
STAT- MEDLINE
DCOM- 20080409
LR  - 20171116
IS  - 0959-4965 (Print)
IS  - 0959-4965 (Linking)
VI  - 19
IP  - 3
DP  - 2008 Feb 12
TI  - Type 2 diabetes with neuropathy: autoantibody stimulation of autophagy via Fas.
PG  - 265-9
LID - 10.1097/WNR.0b013e3282f4cb50 [doi]
AB  - We reported previously that sera from patients with type 2 diabetes and 
      neuropathy induce autophagy in human neuroblastoma (SH-SY5Y) cells. Here we 
      report that enriched immunoglobulin fractions from a subpopulation of these 
      patients induce autophagy and colocalization with Fas-activated death domain 
      (FADD), a component of the Fas-activated death domain receptor signaling pathway. 
      These effects were replicated by treatment of SY5Y cells with Fas ligand, tumor 
      necrosis factor alpha and an agonist anti-Fas antibody. Preincubation of these 
      sera with a soluble Fas receptor chimera (extracellular domain) markedly 
      decreased the stimulation of autophagy. The results suggest that sera from subset 
      of individuals with type 2 diabetes and neuropathy contain autoantibodies that 
      activate the Fas cascade.
FAU - Towns, Roberto
AU  - Towns R
AD  - Department of Internal Medicine, University of Michigan Health System, Ann Arbor, 
      Michigan, USA.
FAU - Guo, Chunfang
AU  - Guo C
FAU - Shangguan, Yu
AU  - Shangguan Y
FAU - Hong, Shuangsong
AU  - Hong S
FAU - Wiley, John W
AU  - Wiley JW
LA  - eng
GR  - M01-RR-00042/RR/NCRR NIH HHS/United States
GR  - R01-052387/PHS HHS/United States
GR  - R01-056997/PHS HHS/United States
GR  - RR024986/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Autoantibodies)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 0 (fas Receptor)
SB  - IM
MH  - Adult
MH  - Autoantibodies/*immunology
MH  - Autophagy/*genetics/*physiology
MH  - Blotting, Western
MH  - Cell Line, Tumor
MH  - Diabetes Mellitus, Type 2/*immunology/*pathology
MH  - Diabetic Neuropathies/*immunology/*pathology
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Immunoglobulin G/biosynthesis/genetics
MH  - Immunoglobulin M/biosynthesis/genetics
MH  - Male
MH  - Neuroblastoma/pathology
MH  - fas Receptor/genetics/*physiology
EDAT- 2008/02/28 09:00
MHDA- 2008/04/10 09:00
CRDT- 2008/02/28 09:00
PHST- 2008/02/28 09:00 [pubmed]
PHST- 2008/04/10 09:00 [medline]
PHST- 2008/02/28 09:00 [entrez]
AID - 00001756-200802120-00002 [pii]
AID - 10.1097/WNR.0b013e3282f4cb50 [doi]
PST - ppublish
SO  - Neuroreport. 2008 Feb 12;19(3):265-9. doi: 10.1097/WNR.0b013e3282f4cb50.