PMID- 18304466
OWN - NLM
STAT- MEDLINE
DCOM- 20080408
LR  - 20181201
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 121
IP  - 4
DP  - 2008 Feb 20
TI  - Effect of all-trans retinoic acid on drug sensitivity and expression of survivin 
      in LoVo cells.
PG  - 331-5
AB  - BACKGROUND: All-trans retinoic acid (ATRA) can influence the tumor cell 
      proliferation cycle, and some chemotherapeutic drugs are cycle specific. In this 
      study, we hypothesize that ATRA can enhance chemotherapeutic drug sensitivity by 
      affecting the cell cycle of tumor cells. METHODS: The cell cycle of LoVo cells 
      was evaluated using flow cytometry (FCM). Cell viability was analyzed using the 
      MTT assay. The morphologic changes in the treated LoVo cells were measured with 
      acridine orange (AO)/ethidium bromide (EB) staining. Expression of survivin in 
      LoVo cells was analyzed by immunofluorescence assay. RESULTS: After LoVo cells 
      were treated with ATRA, the G0/G1 ratio of the tumor cells increased and the cell 
      ratio of S- and G2/M-phase decreased. Viability of the cells decreased 
      significantly after combined treatment with ATRA and 5-fluorouracil (5-FU) or 
      mitomycin c (MMC) and was evaluated by fluorescence microscopy. Expression level 
      of survivin in the tumor cells decreased after ATRA combination treatment. 
      CONCLUSIONS: ATRA enhances drug sensitivity of the LoVo cell line to cell 
      cycle-specific agents and inhibits the expression of survivin in LoVo cells. The 
      combination of ATRA and 5-FU or MMC promoted cell apoptosis, and the mechanism 
      involved in apoptosis may be related to inhibition of survivin gene expression.
FAU - Wei, Hong-bo
AU  - Wei HB
AD  - Department of Gastrointestinal Surgery, Third Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou, Guangdong 510630, China. drwhb@21cn.com
FAU - Hu, Bao-guang
AU  - Hu BG
FAU - Han, Xiao-yan
AU  - Han XY
FAU - Zheng, Zong-heng
AU  - Zheng ZH
FAU - Wei, Bo
AU  - Wei B
FAU - Huang, Jiang-long
AU  - Huang JL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (BIRC5 protein, human)
RN  - 0 (Inhibitor of Apoptosis Proteins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Survivin)
RN  - 5688UTC01R (Tretinoin)
SB  - IM
MH  - Cell Line, Tumor
MH  - Colonic Neoplasms/drug therapy/metabolism
MH  - Drug Resistance, Neoplasm
MH  - Flow Cytometry
MH  - Gene Expression Regulation, Neoplastic/*drug effects
MH  - Humans
MH  - Inhibitor of Apoptosis Proteins
MH  - Microtubule-Associated Proteins/*genetics
MH  - Neoplasm Proteins/*genetics
MH  - Survivin
MH  - Tretinoin/*pharmacology
EDAT- 2008/02/29 09:00
MHDA- 2008/04/09 09:00
CRDT- 2008/02/29 09:00
PHST- 2008/02/29 09:00 [pubmed]
PHST- 2008/04/09 09:00 [medline]
PHST- 2008/02/29 09:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 2008 Feb 20;121(4):331-5.