PMID- 18304588
OWN - NLM
STAT- MEDLINE
DCOM- 20081001
LR  - 20080616
IS  - 0025-326X (Print)
IS  - 0025-326X (Linking)
VI  - 57
IP  - 6-12
DP  - 2008
TI  - Molecular cloning and beta-naphthoflavone-induced expression of a cytochrome P450 
      1A (CYP1A) gene from an anadromous river pufferfish, Takifugu obscurus.
PG  - 433-40
LID - 10.1016/j.marpolbul.2008.01.005 [doi]
AB  - In recent years, there has been a decline in the wild populations of river 
      pufferfish, Takifugu obscurus. Besides overexploitation for commercial purposes, 
      environmental pollution is believed to have contributed to its decline. However, 
      almost no information exists about genes involved in metabolism of xenobiotics by 
      this species. Nevertheless, there is interest in fugu fishes, since they possess 
      the smallest genome among vertebrates. We cloned and characterized the 
      full-length cDNA sequence of a cytochrome P450 1A (CYP1A) gene from T. obscurus. 
      Phylogenic relationship of T. obscurus CYP1A was also compared to other fish 
      species. The tissue distribution and time-dependant induction of CYP1A mRNA were 
      studied by real-time PCR in T. obscurus exposed to an aryl hydrocarbon receptor 
      (Ahr) agonist, beta-naphthoflavone (BNF). The greatest basal expression in livers 
      of control as well as BNF-treated individuals. However, brain, gill, gonad, 
      intestine, and kidney also expressed CYP1A. Muscles expressed the least CYP1A. 
      The results of the time-course study revealed induction in brain and gills after 
      6h and at 12h in most tissues. Except for gills, all other organs retained 
      induced expression of CYP1A mRNA up to 96h.
FAU - Kim, Jin-Hyoung
AU  - Kim JH
AD  - Department of Marine Science, College of Natural Sciences, Inha University, 
      Incheon 402-751, South Korea.
FAU - Raisuddin, Sheikh
AU  - Raisuddin S
FAU - Ki, Jang-Seu
AU  - Ki JS
FAU - Lee, Jae-Seong
AU  - Lee JS
FAU - Han, Kyung-Nam
AU  - Han KN
LA  - eng
SI  - GENBANK/EF584508
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080304
PL  - England
TA  - Mar Pollut Bull
JT  - Marine pollution bulletin
JID - 0260231
RN  - 0 (Water Pollutants, Chemical)
RN  - 6051-87-2 (beta-Naphthoflavone)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Cytochrome P-450 CYP1A1/chemistry/*genetics
MH  - Gene Expression Regulation, Enzymologic/*drug effects
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Takifugu/classification/*genetics/*metabolism
MH  - Water Pollutants, Chemical/toxicity
MH  - beta-Naphthoflavone/*toxicity
EDAT- 2008/02/29 09:00
MHDA- 2008/10/02 09:00
CRDT- 2008/02/29 09:00
PHST- 2007/07/31 00:00 [received]
PHST- 2008/01/05 00:00 [accepted]
PHST- 2008/02/29 09:00 [pubmed]
PHST- 2008/10/02 09:00 [medline]
PHST- 2008/02/29 09:00 [entrez]
AID - S0025-326X(08)00016-7 [pii]
AID - 10.1016/j.marpolbul.2008.01.005 [doi]
PST - ppublish
SO  - Mar Pollut Bull. 2008;57(6-12):433-40. doi: 10.1016/j.marpolbul.2008.01.005. Epub 
      2008 Mar 4.