PMID- 18304723
OWN - NLM
STAT- MEDLINE
DCOM- 20080507
LR  - 20131121
IS  - 0301-4622 (Print)
IS  - 0301-4622 (Linking)
VI  - 134
IP  - 1-2
DP  - 2008 Apr
TI  - DNA binding and photocleavage properties of a novel cationic 
      porphyrin-anthraquinone hybrid.
PG  - 72-83
LID - 10.1016/j.bpc.2008.01.009 [doi]
AB  - A novel cationic porphyrin-anthraquinone (Por-AQ) hybrid has been synthesized and 
      characterized. Using the combination of absorption titration, fluorescence 
      spectra, circular dichroism (CD) as well as viscosity measurements, the binding 
      properties of the hybrid to calf thymus (CT) DNA have been investigated compared 
      with its parent porphyrin. The experimental results show that at low [Por]/[DNA] 
      ratios, the parent porphyrin binds to DNA in an intercalative mode while the 
      hybrid binds in a combined mode of outside binding (for porphyrin moiety) and 
      partial intercalation (for anthraquinone). Ethidium bromide (EB) competition 
      experiment determined the binding affinity constants (K(app)) of the compounds 
      for CT DNA. Theoretical calculational results applying the density functional 
      theory (DFT) can explain the different DNA binding behaviors reasonably. (1)O(2) 
      was suggested to be the reactive species responsible for the DNA photocleavage of 
      porphyrin moieties in both two compounds. The wavelength-depending cleavage 
      activities of the compounds were also investigated.
FAU - Zhao, Ping
AU  - Zhao P
AD  - MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and 
      Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
FAU - Xu, Lian-Cai
AU  - Xu LC
FAU - Huang, Jin-Wang
AU  - Huang JW
FAU - Zheng, Kang-Cheng
AU  - Zheng KC
FAU - Liu, Jie
AU  - Liu J
FAU - Yu, Han-Cheng
AU  - Yu HC
FAU - Ji, Liang-Nian
AU  - Ji LN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080209
PL  - Netherlands
TA  - Biophys Chem
JT  - Biophysical chemistry
JID - 0403171
RN  - 0 (Anthraquinones)
RN  - 0 (Intercalating Agents)
RN  - 0 (Porphyrins)
RN  - 9007-49-2 (DNA)
RN  - EN464416SI (Ethidium)
SB  - IM
MH  - Absorption
MH  - Animals
MH  - Anthraquinones/chemistry/*pharmacology
MH  - Binding, Competitive
MH  - Cattle
MH  - Circular Dichroism
MH  - DNA/genetics/*metabolism
MH  - DNA Cleavage/*drug effects/radiation effects
MH  - Drug Design
MH  - Ethidium/chemistry/pharmacology
MH  - Intercalating Agents/chemistry/pharmacology
MH  - Models, Chemical
MH  - Photolysis
MH  - Porphyrins/chemistry/*pharmacology
MH  - Spectrometry, Fluorescence
MH  - Titrimetry
MH  - Viscosity
EDAT- 2008/02/29 09:00
MHDA- 2008/05/08 09:00
CRDT- 2008/02/29 09:00
PHST- 2007/11/09 00:00 [received]
PHST- 2008/01/19 00:00 [revised]
PHST- 2008/01/19 00:00 [accepted]
PHST- 2008/02/29 09:00 [pubmed]
PHST- 2008/05/08 09:00 [medline]
PHST- 2008/02/29 09:00 [entrez]
AID - S0301-4622(08)00022-7 [pii]
AID - 10.1016/j.bpc.2008.01.009 [doi]
PST - ppublish
SO  - Biophys Chem. 2008 Apr;134(1-2):72-83. doi: 10.1016/j.bpc.2008.01.009. Epub 2008 
      Feb 9.