PMID- 18304740
OWN - NLM
STAT- MEDLINE
DCOM- 20080721
LR  - 20211020
IS  - 0306-4522 (Print)
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 152
IP  - 3
DP  - 2008 Mar 27
TI  - Schwann cells genetically modified to express neurotrophins promote spiral 
      ganglion neuron survival in vitro.
PG  - 821-8
LID - 10.1016/j.neuroscience.2007.11.057 [doi]
AB  - The intracochlear infusion of neurotrophic factors via a mini-osmotic pump has 
      been shown to prevent deafness-induced spiral ganglion neuron (SGN) degeneration; 
      however, the use of pumps may increase the incidence of infection within the 
      cochlea, making this technique unsuitable for neurotrophin administration in a 
      clinical setting. Cell- and gene-based therapies are potential therapeutic 
      options. This study investigated whether Schwann cells which were genetically 
      modified to over-express the neurotrophins brain-derived neurotrophic factor 
      (BDNF) or neurotrophin 3 (Ntf3, formerly NT-3) could support SGN survival in an 
      in vitro model of deafness. Co-culture of either BDNF over-expressing Schwann 
      cells or Ntf3 over-expressing Schwann cells with SGNs from early postnatal rats 
      significantly enhanced neuronal survival in comparison to both control Schwann 
      cells and conventional recombinant neurotrophin proteins. Transplantation of 
      neurotrophin over-expressing Schwann cells into the cochlea may provide an 
      alternative means of delivering neurotrophic factors to the deaf cochlea for 
      therapeutic purposes.
FAU - Pettingill, L N
AU  - Pettingill LN
AD  - The Bionic Ear Institute, 384 Albert Street, East Melbourne, Australia 3002. 
      lpettingill@bionicear.org
FAU - Minter, R L
AU  - Minter RL
FAU - Shepherd, R K
AU  - Shepherd RK
LA  - eng
GR  - N01 DC031005/DC/NIDCD NIH HHS/United States
GR  - N01-DC-3-1005/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080101
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Cell Communication/genetics
MH  - Cell Survival/*genetics
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Gene Expression Regulation/genetics
MH  - Genetic Therapy/methods
MH  - Hearing Loss, Sensorineural/genetics/prevention & control/therapy
MH  - Nerve Degeneration/genetics/prevention & control/therapy
MH  - Nerve Growth Factors/*genetics/metabolism
MH  - Neurons, Afferent/cytology/*metabolism
MH  - Neurotrophin 3/genetics/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Schwann Cells/cytology/*metabolism
MH  - Spiral Ganglion/cytology/*metabolism
PMC - PMC2726277
MID - NIHMS46172
EDAT- 2008/02/29 09:00
MHDA- 2008/07/22 09:00
PMCR- 2009/08/13
CRDT- 2008/02/29 09:00
PHST- 2007/08/30 00:00 [received]
PHST- 2007/11/26 00:00 [revised]
PHST- 2008/01/09 00:00 [accepted]
PHST- 2008/02/29 09:00 [pubmed]
PHST- 2008/07/22 09:00 [medline]
PHST- 2008/02/29 09:00 [entrez]
PHST- 2009/08/13 00:00 [pmc-release]
AID - S0306-4522(07)01528-X [pii]
AID - 10.1016/j.neuroscience.2007.11.057 [doi]
PST - ppublish
SO  - Neuroscience. 2008 Mar 27;152(3):821-8. doi: 10.1016/j.neuroscience.2007.11.057. 
      Epub 2008 Jan 1.