PMID- 18306107
OWN - NLM
STAT- MEDLINE
DCOM- 20080403
LR  - 20191110
IS  - 1532-2513 (Electronic)
IS  - 0892-3973 (Linking)
VI  - 30
IP  - 1
DP  - 2008
TI  - PPD extract induces the maturation of human monocyte-derived dendritic cells.
PG  - 91-104
LID - 10.1080/08923970701812654 [doi]
AB  - Dendritic cells (DCs) are the most powerful antigen presenting cells, capable of 
      inducing T-dependent immune responses even in naive T cells. DCs are of special 
      interest as cellular adjuvants for immunity induction in clinical settings and 
      several methods for their generation and maturation are recently under 
      investigation. The present study was set out to define the effects of PPD 
      (Purified Protein Derivative), a mycobacterial extract used in the tuberculin 
      skin test, on in vitro differentiation and maturation of human monocyte derived 
      dendritic cells. Immature DCs were prepared from the peripheral blood monocytes 
      of healthy volunteers by culturing in a medium supplemented with granulocyte 
      macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4). The 
      resultant cells were then stimulated with PPD extract and their properties such 
      as cell morphology and the expression of key surface molecules were compared with 
      tumor necrosis factor-alpha (TNF-alpha) stimulated immature DCs. Our results 
      suggest that mycobacterial purified extract is as potent as TNF-alpha, a 
      well-established DC stimulator, in induction of maturation in human monocyte 
      derived DCs. We also ruled out the contribution of lipopolysaccharide (LPS) and 
      beta-glucan contamination in maturation effect of PPD preparations. So, PPD as an 
      examined safe material for in vivo consumption could be used to stimulate DC 
      maturation in DC based immunotherapy protocols.
FAU - Bagheri, Kambiz
AU  - Bagheri K
AD  - Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares 
      University, Tehran, Iran.
FAU - Delirezh, Nowruz
AU  - Delirezh N
FAU - Moazzeni, Seyed-Mohammad
AU  - Moazzeni SM
LA  - eng
PT  - Journal Article
PL  - England
TA  - Immunopharmacol Immunotoxicol
JT  - Immunopharmacology and immunotoxicology
JID - 8800150
RN  - 0 (Endotoxins)
RN  - 0 (Tuberculin)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (beta-Glucans)
SB  - IM
MH  - Cell Differentiation
MH  - Dendritic Cells/cytology/*drug effects/*immunology
MH  - Endotoxins/pharmacology
MH  - Humans
MH  - Immunotherapy/*methods
MH  - Monocytes/immunology
MH  - Tuberculin/*pharmacology
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - beta-Glucans/pharmacology
EDAT- 2008/02/29 09:00
MHDA- 2008/04/04 09:00
CRDT- 2008/02/29 09:00
PHST- 2008/02/29 09:00 [pubmed]
PHST- 2008/04/04 09:00 [medline]
PHST- 2008/02/29 09:00 [entrez]
AID - 791005424 [pii]
AID - 10.1080/08923970701812654 [doi]
PST - ppublish
SO  - Immunopharmacol Immunotoxicol. 2008;30(1):91-104. doi: 10.1080/08923970701812654.