PMID- 18308859
OWN - NLM
STAT- MEDLINE
DCOM- 20080522
LR  - 20201209
IS  - 0193-1857 (Print)
IS  - 0193-1857 (Linking)
VI  - 294
IP  - 4
DP  - 2008 Apr
TI  - A new possibility for repairing the anal dysfunction by promoting regeneration of 
      the reflex pathways in the enteric nervous system.
PG  - G1084-93
LID - 10.1152/ajpgi.00345.2007 [doi]
AB  - Moderate rectal distension elicits recto-rectal reflex contractions and 
      simultaneous recto-internal anal sphincter reflex relaxations that together 
      comprise the defecation reflex. Both reflexes are controlled by 1) pelvic nerves, 
      2) lumbar colonic nerves, and 3) enteric nervous system. The aim of the present 
      study was to explore a novel approach to repairing the defecation reflex 
      dysfunction by using the plasticity of enteric nervous pathways. Experiments were 
      performed in anesthetized guinea pigs with ethyl carbamate. The rectum 30 mm oral 
      from the anal verge was transected without damage to extrinsic nerves, and 
      subsequent end-to-end one-layer anastomosis was performed. Recovery of the 
      defecation reflex and associated reflex pathways were evaluated. Eight weeks 
      after sectioning of intrinsic reflex nerve pathways in the rectum, the defecation 
      reflex recovered to the control level, accompanied with regeneration of reflex 
      pathways. The 5-HT(4)-receptor agonist mosapride (0.5 and 1.0 mg/kg) 
      significantly (P < 0.01) enhanced the recovered defecation reflex 8 wk after 
      surgery. Two weeks after local treatment with brain-derived neurotrophic factor 
      (BDNF: 10(-6) g/ml) at the rectal anastomotic site, the recto-internal anal 
      sphincter reflex relaxations recovered and some bundles of fine nerve fibers were 
      shown to interconnect the oral and anal ends of the myenteric plexus. These 
      results suggested a possibility for repairing the anal dysfunction by promoting 
      regeneration of the reflex pathways in the enteric nervous system with local 
      application of BDNF.
FAU - Katsui, Renta
AU  - Katsui R
AD  - Dept. of Physiology II, Nara Medical Univ., 840 Shijo-cho, Kashihara 634-8521, 
      Japan.
FAU - Kojima, Yu
AU  - Kojima Y
FAU - Kuniyasu, Hiroki
AU  - Kuniyasu H
FAU - Shimizu, Juichiro
AU  - Shimizu J
FAU - Koyama, Fumikazu
AU  - Koyama F
FAU - Fujii, Hisao
AU  - Fujii H
FAU - Nakajima, Yoshiyuki
AU  - Nakajima Y
FAU - Takaki, Miyako
AU  - Takaki M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080228
PL  - United States
TA  - Am J Physiol Gastrointest Liver Physiol
JT  - American journal of physiology. Gastrointestinal and liver physiology
JID - 100901227
RN  - 0 (Benzamides)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Distal-less homeobox proteins)
RN  - 0 (Gastrointestinal Agents)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Morpholines)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurofilament Proteins)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (Receptors, Growth Factor)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 0 (Serotonin 5-HT4 Receptor Agonists)
RN  - 0 (Serotonin Receptor Agonists)
RN  - 0 (Transcription Factors)
RN  - 136958-07-1 (Ngfr protein, rat)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - I8MFJ1C0BY (mosapride)
SB  - IM
MH  - Anal Canal/*innervation/physiopathology/surgery
MH  - Anastomosis, Surgical
MH  - Animals
MH  - Benzamides/*pharmacology
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Cell Proliferation
MH  - *Defecation/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Enteric Nervous System/drug effects/metabolism/*physiopathology/surgery
MH  - Gastrointestinal Agents/pharmacology
MH  - Guinea Pigs
MH  - Homeodomain Proteins/metabolism
MH  - Immunohistochemistry
MH  - Male
MH  - Morpholines/*pharmacology
MH  - *Nerve Regeneration/drug effects
MH  - Nerve Tissue Proteins
MH  - Neurofilament Proteins/metabolism
MH  - *Neuronal Plasticity/drug effects
MH  - Pressure
MH  - Proliferating Cell Nuclear Antigen/metabolism
MH  - Proto-Oncogene Proteins c-kit/metabolism
MH  - Receptor, trkB/metabolism
MH  - Receptors, Growth Factor
MH  - Receptors, Nerve Growth Factor/metabolism
MH  - Recovery of Function
MH  - Rectum/*innervation/physiopathology/surgery
MH  - *Reflex/drug effects
MH  - Serotonin 5-HT4 Receptor Agonists
MH  - Serotonin Receptor Agonists/pharmacology
MH  - Time Factors
MH  - Transcription Factors/metabolism
EDAT- 2008/03/01 09:00
MHDA- 2008/05/23 09:00
CRDT- 2008/03/01 09:00
PHST- 2008/03/01 09:00 [pubmed]
PHST- 2008/05/23 09:00 [medline]
PHST- 2008/03/01 09:00 [entrez]
AID - 00345.2007 [pii]
AID - 10.1152/ajpgi.00345.2007 [doi]
PST - ppublish
SO  - Am J Physiol Gastrointest Liver Physiol. 2008 Apr;294(4):G1084-93. doi: 
      10.1152/ajpgi.00345.2007. Epub 2008 Feb 28.