PMID- 18309046 OWN - NLM STAT- MEDLINE DCOM- 20080402 LR - 20240109 IS - 1095-9203 (Electronic) IS - 0036-8075 (Print) IS - 0036-8075 (Linking) VI - 319 IP - 5870 DP - 2008 Mar 21 TI - Protein synthesis and neurotrophin-dependent structural plasticity of single dendritic spines. PG - 1683-7 LID - 10.1126/science.1152864 [doi] AB - Long-term potentiation (LTP) at glutamatergic synapses is considered to underlie learning and memory and is associated with the enlargement of dendritic spines. Because the consolidation of memory and LTP require protein synthesis, it is important to clarify how protein synthesis affects spine enlargement. In rat brain slices, the repetitive pairing of postsynaptic spikes and two-photon uncaging of glutamate at single spines (a spike-timing protocol) produced both immediate and gradual phases of spine enlargement in CA1 pyramidal neurons. The gradual enlargement was strongly dependent on protein synthesis and brain-derived neurotrophic factor (BDNF) action, often associated with spine twitching, and was induced specifically at the spines that were immediately enlarged by the synaptic stimulation. Thus, this spike-timing protocol is an efficient trigger for BDNF secretion and induces protein synthesis-dependent long-term enlargement at the level of single spines. FAU - Tanaka, Jun-Ichi AU - Tanaka J AD - Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 113-0033, Japan. FAU - Horiike, Yoshihiro AU - Horiike Y FAU - Matsuzaki, Masanori AU - Matsuzaki M FAU - Miyazaki, Takashi AU - Miyazaki T FAU - Ellis-Davies, Graham C R AU - Ellis-Davies GC FAU - Kasai, Haruo AU - Kasai H LA - eng GR - R01 GM053395/GM/NIGMS NIH HHS/United States GR - R01 GM053395-12/GM/NIGMS NIH HHS/United States GR - R24 GM065473/GM/NIGMS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20080228 PL - United States TA - Science JT - Science (New York, N.Y.) JID - 0404511 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Protein Synthesis Inhibitors) RN - 3KX376GY7L (Glutamic Acid) RN - EC 2.7.10.1 (Receptor, trkB) SB - IM EIN - Science. 2009 Dec 11;326(5959):1482 CIN - Science. 2008 Mar 21;319(5870):1627-8. PMID: 18356512 MH - Action Potentials MH - Animals MH - Brain-Derived Neurotrophic Factor/*metabolism/pharmacology MH - Cells, Cultured MH - Dendritic Spines/*physiology/*ultrastructure MH - Glutamic Acid/metabolism MH - *Neuronal Plasticity MH - Patch-Clamp Techniques MH - *Protein Biosynthesis MH - Protein Synthesis Inhibitors/pharmacology MH - Pyramidal Cells/physiology/ultrastructure MH - Rats MH - Rats, Sprague-Dawley MH - Receptor, trkB/metabolism MH - Synapses/*physiology PMC - PMC4218863 MID - NIHMS304045 EDAT- 2008/03/01 09:00 MHDA- 2008/04/03 09:00 PMCR- 2014/11/03 CRDT- 2008/03/01 09:00 PHST- 2008/03/01 09:00 [pubmed] PHST- 2008/04/03 09:00 [medline] PHST- 2008/03/01 09:00 [entrez] PHST- 2014/11/03 00:00 [pmc-release] AID - 1152864 [pii] AID - 10.1126/science.1152864 [doi] PST - ppublish SO - Science. 2008 Mar 21;319(5870):1683-7. doi: 10.1126/science.1152864. Epub 2008 Feb 28.