PMID- 18309356
OWN - NLM
STAT- MEDLINE
DCOM- 20081028
LR  - 20171116
IS  - 1751-7362 (Print)
IS  - 1751-7362 (Linking)
VI  - 2
IP  - 5
DP  - 2008 May
TI  - Substrate-induced growth and isolation of Acidobacteria from acidic Sphagnum 
      peat.
PG  - 551-60
LID - 10.1038/ismej.2008.7 [doi]
AB  - Fluorescence in situ hybridization (FISH) was applied to estimate the population 
      size of the poorly characterized phylum Acidobacteria in acidic peat sampled from 
      nine different Sphagnum-dominated wetlands of Northern Russia. The cell numbers 
      of these bacteria in oxic peat layers ranged from 0.4 x 10(6) to 1.3 x 10(7) 
      cells per g of wet peat, comprising up to 4% of total bacterial cells. 
      Substrate-induced growth of acidobacteria was observed after amendment of peat 
      samples with glucose, pectin, xylan, starch, ethanol and methanol, while weak or 
      no response was obtained for acetate, pyruvate, mannitol and cellobiose. Using 
      low-nutrient media and FISH-mediated monitoring of the isolation procedure, we 
      succeeded in obtaining nine strains of acidobacteria in pure cultures. These 
      strains belonged to subdivisions 1 and 3 of the Acidobacteria and represented 
      strictly aerobic, heterotrophic organisms. Except for methanol, the substrate 
      utilization patterns of these isolates matched the results obtained in our 
      substrate-amendment experiments with native peat. All strains were also capable 
      of utilizing galacturonic acid, a characteristic component of the cell wall in 
      Sphagnum spp, which is released during moss decomposition. Most isolates from 
      subdivision 1 were truly acidophilic organisms with the growth optimum at pH 
      3.5-4.5, while the isolates from subdivision 3 grew optimally at pH 5.5-6.5. 
      Another important phenotypic trait of novel strains was their capability of 
      active growth at low temperatures. Both acidophily and low-temperature growth are 
      consistent with the occurrence of acidobacteria in cold and acidic northern 
      wetlands.
FAU - Pankratov, Timofei A
AU  - Pankratov TA
AD  - Department of Microbial Communities, SN Winogradsky Institute of Microbiology, 
      Russian Academy of Sciences, Moscow, Russia.
FAU - Serkebaeva, Yulia M
AU  - Serkebaeva YM
FAU - Kulichevskaya, Irina S
AU  - Kulichevskaya IS
FAU - Liesack, Werner
AU  - Liesack W
FAU - Dedysh, Svetlana N
AU  - Dedysh SN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080228
PL  - England
TA  - ISME J
JT  - The ISME journal
JID - 101301086
RN  - 0 (Soil)
SB  - IM
MH  - Bacteria/*metabolism
MH  - Hydrogen-Ion Concentration
MH  - In Situ Hybridization, Fluorescence
MH  - Phylogeny
MH  - *Soil
MH  - *Soil Microbiology
MH  - *Sphagnopsida
EDAT- 2008/03/01 09:00
MHDA- 2008/10/29 09:00
CRDT- 2008/03/01 09:00
PHST- 2008/03/01 09:00 [pubmed]
PHST- 2008/10/29 09:00 [medline]
PHST- 2008/03/01 09:00 [entrez]
AID - ismej20087 [pii]
AID - 10.1038/ismej.2008.7 [doi]
PST - ppublish
SO  - ISME J. 2008 May;2(5):551-60. doi: 10.1038/ismej.2008.7. Epub 2008 Feb 28.