PMID- 18310438
OWN - NLM
STAT- MEDLINE
DCOM- 20080519
LR  - 20211020
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 74
IP  - 9
DP  - 2008 May
TI  - Temporal transcriptomic microarray analysis of "Dehalococcoides ethenogenes" 
      strain 195 during the transition into stationary phase.
PG  - 2864-72
LID - 10.1128/AEM.02208-07 [doi]
AB  - "Dehalococcoides" bacteria can reductively dehalogenate a wide range of 
      halogenated organic pollutants. In this study, DNA microarrays were used to 
      monitor dynamic changes in the transcriptome as "Dehalococcoides ethenogenes" 
      strain 195 transitioned from exponential growth into stationary phase. In total, 
      415 nonredundant genes were identified as differentially expressed. As expected, 
      genes involved with translation and energy metabolism were down-regulated while 
      genes involved with general stress response, transcription, and signal 
      transduction were up-regulated. Unexpected, however, was the 8- to 10-fold 
      up-regulation of four putative reductive dehalogenases (RDases) (DET0173, 
      DET0180, DET1535, and DET1545). Another unexpected finding was the up-regulation 
      of a large number of genes located within integrated elements, including a 
      putative prophage and a multicopy transposon. Finally, genes encoding the 
      dominant hydrogenase-RDase respiratory chain of this strain (Hup and TceA) were 
      expressed at stable levels throughout the experiment, providing molecular 
      evidence that strain 195 can uncouple dechlorination from net growth.
FAU - Johnson, David R
AU  - Johnson DR
AD  - Department of Civil and Environmental Engineering, University of California, 
      Berkeley, CA 94720-1710, USA.
FAU - Brodie, Eoin L
AU  - Brodie EL
FAU - Hubbard, Alan E
AU  - Hubbard AE
FAU - Andersen, Gary L
AU  - Andersen GL
FAU - Zinder, Stephen H
AU  - Zinder SH
FAU - Alvarez-Cohen, Lisa
AU  - Alvarez-Cohen L
LA  - eng
SI  - GEO/GPL6336
SI  - GEO/GSE10185
GR  - P42 ES004705/ES/NIEHS NIH HHS/United States
GR  - ES04705/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20080229
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA Transposable Elements)
RN  - 0 (Enzymes)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Bacterial Proteins/genetics
MH  - Chloroflexi/*genetics/*growth & development
MH  - DNA Transposable Elements
MH  - Down-Regulation
MH  - Energy Metabolism/genetics
MH  - Enzymes/genetics
MH  - *Gene Expression Profiling
MH  - *Microarray Analysis
MH  - Prophages/genetics
MH  - Protein Biosynthesis
MH  - Signal Transduction/genetics
MH  - Time Factors
MH  - Transcription Factors/genetics
PMC - PMC2394897
EDAT- 2008/03/04 09:00
MHDA- 2008/05/20 09:00
PMCR- 2008/11/01
CRDT- 2008/03/04 09:00
PHST- 2008/03/04 09:00 [pubmed]
PHST- 2008/05/20 09:00 [medline]
PHST- 2008/03/04 09:00 [entrez]
PHST- 2008/11/01 00:00 [pmc-release]
AID - AEM.02208-07 [pii]
AID - 2208-07 [pii]
AID - 10.1128/AEM.02208-07 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2008 May;74(9):2864-72. doi: 10.1128/AEM.02208-07. Epub 
      2008 Feb 29.