PMID- 18312594
OWN - NLM
STAT- MEDLINE
DCOM- 20080730
LR  - 20080515
IS  - 1744-313X (Electronic)
IS  - 1744-3121 (Linking)
VI  - 35
IP  - 3
DP  - 2008 Jun
TI  - Characterization, expression and evolution analysis of Toll-like receptor 1 gene 
      in pufferfish (Tetraodon nigroviridis).
PG  - 215-25
LID - 10.1111/j.1744-313X.2008.00759.x [doi]
AB  - The Toll-like receptor (TLR) family is a pathogen-associated molecular pattern 
      recognition receptor family that plays key roles in the activation of innate 
      immune system. As an important member of TLRs, TLR1 is involved in the 
      recognition of triacylated lipoproteins and mycobacterial products by binding to 
      TLR2 to form a heterodimer. Although partial sequences of TLR1 has been predicted 
      in Fugu and Danio by bioinformatics, the molecular cloning and characterization 
      of this gene in fish still remains largely unclear. Here we report the 
      full-length sequence of TLR1 gene in the pufferfish (Tetraodon nigroviridis), 
      providing experimental evidence to its existence in fish. The cloned Tetraodon 
      TLR1 (TnTLR1) cDNA exhibited 2587 bp in length and contains a 122-bp 5' 
      untranslated region (UTR), a 2391-bp open reading frame and a 74-bp 3' UTR. 
      Expression of TLR1 transcripts in most selected tissues were detected at lower 
      levels; however, the transcripts in spleen was markedly increased (threefold) 
      after stimulation with lipopolysaccharide, indicating that TLR1 in fish may be 
      involved in the inflammatory responses to bacterial endotoxin or Gram-negative 
      bacteria infection. Gene organization of TnTLR1 is similar to mammalian and 
      avian, and a high degree of conservation of chromosome synteny exists between 
      them. The protein sequence shares 33.7-77.3% identity with known TLR1 members. 
      Phylogenetic analysis grouped TnTLR1 with other fish TLRs on a separated branch, 
      excluded from mammalian and avian TLR1s. TnTLR1 with 796 amino acids has a 
      calculated molecular mass of 90.69 kDa and a theoretical pI of 8.43. 
      Structurally, TnTLR1 has a Toll/interleukin-1 receptor (TIR) domain, a 
      transmembrane domain and leucine-rich repeat domain which are the hallmarks of 
      TLR family. Phylogenetic analysis provided evidence that the TnTLR1 was very 
      close to mammalian TLR1, TLR6 and TLR10 and seemed to be their common ancestor. 
      This report of the full-length TLR1 gene in Tetraodon will provide us further 
      insights into the study of both function and evolution of fish TLRs as a whole.
FAU - Wu, X-Y
AU  - Wu XY
AD  - College of Life Sciences, Zhejiang University, Hangzhou, China.
FAU - Xiang, L-X
AU  - Xiang LX
FAU - Huang, L
AU  - Huang L
FAU - Jin, Y
AU  - Jin Y
FAU - Shao, J-Z
AU  - Shao JZ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080228
PL  - England
TA  - Int J Immunogenet
JT  - International journal of immunogenetics
JID - 101232337
RN  - 0 (Toll-Like Receptor 1)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Computational Biology
MH  - Evolution, Molecular
MH  - *Gene Expression
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Protein Structure, Tertiary
MH  - Sequence Alignment
MH  - Synteny
MH  - Tetraodontiformes/*genetics
MH  - Toll-Like Receptor 1/chemistry/*genetics/metabolism
EDAT- 2008/03/04 09:00
MHDA- 2008/07/31 09:00
CRDT- 2008/03/04 09:00
PHST- 2008/03/04 09:00 [pubmed]
PHST- 2008/07/31 09:00 [medline]
PHST- 2008/03/04 09:00 [entrez]
AID - EJI759 [pii]
AID - 10.1111/j.1744-313X.2008.00759.x [doi]
PST - ppublish
SO  - Int J Immunogenet. 2008 Jun;35(3):215-25. doi: 10.1111/j.1744-313X.2008.00759.x. 
      Epub 2008 Feb 28.