PMID- 18313646
OWN - NLM
STAT- MEDLINE
DCOM- 20080801
LR  - 20161124
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 1203
DP  - 2008 Apr 8
TI  - NGF and BDNF expression drop off in neurally differentiated bone marrow stromal 
      stem cells.
PG  - 26-31
LID - 10.1016/j.brainres.2008.01.086 [doi]
AB  - Bone marrow stromal stem cells (BMSC) express two neurotrophins nerve growth 
      factor (NGF) and brain derived growth factor (BDNF) constitutively and can be 
      differentiated into neuronal-like cells and used to treat neural injuries and 
      diseases. The neurotrophins are required for repair of neural tissues. However, 
      it is not evident whether these cells supply the sufficient amounts of the 
      functional growth factors following neuronal differentiation. This study 
      investigates the expression of NGF, BDNF and their processing enzymes Prohormone 
      convertases (PC) Furin, PC5 and PC6 by Real-time RT-PCR during neural 
      differentiation of rat BMSC. The results showed that all inspected processing 
      enzymes are expressed in the cells. The expression of NGF, BDNF and PC5 decreases 
      following differentiation. In addition, BMSCs express Survivin, an anti-apoptotic 
      gene; however, the differentiated cells reduce its expression similar to two 
      neurotrophins, which could make them susceptible to apoptotic death.
FAU - Yaghoobi, Mohammad Mehdi
AU  - Yaghoobi MM
AD  - Department of Biotechnology, Research Institute of Environmental Sciences, 
      International Centre for Science, High Technology & Environmental Sciences, 
      Kerman, Iran. Yaghoobim@icst.ac.ir
FAU - Mahani, Masoud Torkzadeh
AU  - Mahani MT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080213
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Antigens, CD)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Tissue Proteins)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 3.4.21.- (Proprotein Convertases)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Antigens, CD/metabolism
MH  - Bone Marrow Cells/*cytology/physiology
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Flow Cytometry/methods
MH  - Gene Expression Regulation/*physiology
MH  - Nerve Growth Factor/*metabolism
MH  - Nerve Tissue Proteins/metabolism
MH  - Neurons/*physiology
MH  - Proprotein Convertases/metabolism
MH  - Rats
MH  - Stromal Cells/*cytology/physiology
EDAT- 2008/03/04 09:00
MHDA- 2008/08/02 09:00
CRDT- 2008/03/04 09:00
PHST- 2007/11/27 00:00 [received]
PHST- 2007/12/30 00:00 [revised]
PHST- 2008/01/23 00:00 [accepted]
PHST- 2008/03/04 09:00 [pubmed]
PHST- 2008/08/02 09:00 [medline]
PHST- 2008/03/04 09:00 [entrez]
AID - S0006-8993(08)00236-9 [pii]
AID - 10.1016/j.brainres.2008.01.086 [doi]
PST - ppublish
SO  - Brain Res. 2008 Apr 8;1203:26-31. doi: 10.1016/j.brainres.2008.01.086. Epub 2008 
      Feb 13.