PMID- 18314270
OWN - NLM
STAT- MEDLINE
DCOM- 20080710
LR  - 20220331
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 434
IP  - 3
DP  - 2008 Apr 4
TI  - TNF-alpha differentially modulates ion channels of nociceptive neurons.
PG  - 293-8
LID - 10.1016/j.neulet.2008.01.070 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a proinflammatory cytokine involved in 
      the development and maintenance of inflammatory and neuropathic pain conditions. 
      The mechanisms by which TNF-alpha elicits pain behavior are still incompletely 
      understood. Numerous studies suggest that TNF-alpha sensitizes primary afferent 
      neurons. Most recently, it was shown that TNF-alpha induced an enhancement of 
      TTX-R Na(+) current in dorsal root ganglion (DRG) cells. In the present study, we 
      have tested the effect of acute application of TNF-alpha on voltage-gated 
      potassium, calcium and sodium channel currents as well as its influence on 
      membrane conductance in isolated rat DRG neurons. We report that voltage-gated 
      potassium channel currents of nociceptive DRG neurons are not influenced by 
      TNF-alpha (100 ng/ml), while voltage-gated calcium channel currents were 
      decreased voltage-dependently by -7.73+/-6.01% (S.D.), and voltage-activated 
      sodium channels currents were increased by +5.62+/-4.27%, by TNF-alpha. In 
      addition, TNF-alpha induced a significant increase in IV ramps at a potential of 
      +20 mV, which did not exist when the experiments were conducted in a 
      potassium-free solution, indicating that this effect is mainly the result of a 
      change in potassium conductance. These different actions of TNF-alpha might help 
      to explain how it sensitizes primary afferent neurons after nerve injury and thus 
      facilitates pain.
FAU - Czeschik, Johanna Christina
AU  - Czeschik JC
AD  - Institut fur Physiologie, Universitat Duisburg-Essen, Hufelandstrasse 55, 45122 
      Essen, Germany.
FAU - Hagenacker, Tim
AU  - Hagenacker T
FAU - Schafers, Maria
AU  - Schafers M
FAU - Busselberg, Dietrich
AU  - Busselberg D
LA  - eng
PT  - Journal Article
DEP - 20080206
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Calcium Channels)
RN  - 0 (Ion Channels)
RN  - 0 (Potassium Channels)
RN  - 0 (Sodium Channels)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Calcium Channels/drug effects/metabolism
MH  - Cells, Cultured
MH  - Ganglia, Spinal/drug effects/*metabolism
MH  - Hyperalgesia/metabolism/physiopathology
MH  - Ion Channel Gating/drug effects/*physiology
MH  - Ion Channels/drug effects/*metabolism
MH  - Male
MH  - Membrane Potentials/drug effects/physiology
MH  - Neuralgia/metabolism/physiopathology
MH  - Neurons, Afferent/drug effects/*metabolism
MH  - Nociceptors/drug effects/*metabolism
MH  - Potassium Channels/drug effects/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Sodium Channels/drug effects/metabolism
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/*metabolism/pharmacology
EDAT- 2008/03/04 09:00
MHDA- 2008/07/11 09:00
CRDT- 2008/03/04 09:00
PHST- 2007/12/06 00:00 [received]
PHST- 2008/01/04 00:00 [revised]
PHST- 2008/01/29 00:00 [accepted]
PHST- 2008/03/04 09:00 [pubmed]
PHST- 2008/07/11 09:00 [medline]
PHST- 2008/03/04 09:00 [entrez]
AID - S0304-3940(08)00153-5 [pii]
AID - 10.1016/j.neulet.2008.01.070 [doi]
PST - ppublish
SO  - Neurosci Lett. 2008 Apr 4;434(3):293-8. doi: 10.1016/j.neulet.2008.01.070. Epub 
      2008 Feb 6.