PMID- 18317363
OWN - NLM
STAT- MEDLINE
DCOM- 20080819
LR  - 20111117
IS  - 1524-9557 (Print)
IS  - 1524-9557 (Linking)
VI  - 31
IP  - 3
DP  - 2008 Apr
TI  - Detection, isolation, and characterization of alpha-fetoprotein-specific T cell 
      populations and clones using MHC class I multimer magnetic sorting.
PG  - 246-53
LID - 10.1097/CJI.0b013e318169d55c [doi]
AB  - alpha-fetoprotein (AFP) is a fetal protein specifically reexpressed in 50% of 
      hepatocellular carcinomas. This protein could serve as a tumor-associated antigen 
      for immunotherapy purpose. The aim of our work was to analyze the presence of 
      AFP-specific T cell populations in peripheral blood mononuclear cells (PBMCs) 
      from cirrhotic patients with or without hepatocellular carcinoma. Using 
      peptide-major histocompatibility complex class I multimers, AFP-specific 
      populations corresponding to 3 previously described human leukocyte antigen 
      (HLA)-A*0201 major histocompatibility complex class I epitopes (AFP137, AFP158, 
      and AFP325) were sorted magnetically from CD8 positive cells without prior 
      stimulation with the target antigen. T cell populations specific for 1 peptide 
      (AFP158) were frequent, whereas populations corresponding to peptide AFP137 were 
      rare and absent for peptide AFP325. We also isolated and fully characterized T 
      cell clones specific for AFP137 and AFP158 peptides. We show that these clones 
      can be used to monitor dendritic cell loading with peptides and could be useful 
      for future immunotherapy protocols.
FAU - Pichard, Virginie
AU  - Pichard V
AD  - INSERM CIC 04, CHU Hotel Dieu, Nantes, France.
FAU - Royer, Pierre Joseph
AU  - Royer PJ
FAU - Richou, Carine
AU  - Richou C
FAU - Cauchin, Estelle
AU  - Cauchin E
FAU - Goebes, Karen
AU  - Goebes K
FAU - Gaignerie, Anne
AU  - Gaignerie A
FAU - Masliah, Claude
AU  - Masliah C
FAU - Gournay, Jerome
AU  - Gournay J
FAU - Gregoire, Marc
AU  - Gregoire M
FAU - Ferry, Nicolas
AU  - Ferry N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunother
JT  - Journal of immunotherapy (Hagerstown, Md. : 1997)
JID - 9706083
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A*02:01 antigen)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Peptides)
RN  - 0 (alpha-Fetoproteins)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Hepatocellular/immunology/pathology
MH  - Cytotoxicity Tests, Immunologic
MH  - Female
MH  - HLA-A Antigens/*immunology/isolation & purification/metabolism
MH  - HLA-A2 Antigen
MH  - Humans
MH  - *Immunomagnetic Separation
MH  - Leukocytes, Mononuclear/immunology/metabolism/pathology
MH  - Liver Neoplasms/immunology/pathology
MH  - Male
MH  - Middle Aged
MH  - Peptides/chemistry/immunology
MH  - T-Cell Antigen Receptor Specificity
MH  - T-Lymphocyte Subsets/*immunology/pathology
MH  - alpha-Fetoproteins/*analysis/immunology/*metabolism
EDAT- 2008/03/05 09:00
MHDA- 2008/08/20 09:00
CRDT- 2008/03/05 09:00
PHST- 2008/03/05 09:00 [pubmed]
PHST- 2008/08/20 09:00 [medline]
PHST- 2008/03/05 09:00 [entrez]
AID - 00002371-200804000-00003 [pii]
AID - 10.1097/CJI.0b013e318169d55c [doi]
PST - ppublish
SO  - J Immunother. 2008 Apr;31(3):246-53. doi: 10.1097/CJI.0b013e318169d55c.