PMID- 18318993
OWN - NLM
STAT- MEDLINE
DCOM- 20080715
LR  - 20211020
IS  - 1672-7681 (Print)
IS  - 2042-0226 (Electronic)
IS  - 1672-7681 (Linking)
VI  - 5
IP  - 1
DP  - 2008 Feb
TI  - IL-10 gene modified dendritic cells inhibit T helper type 1-mediated alloimmune 
      responses and promote immunological tolerance in diabetes.
PG  - 41-6
LID - 10.1038/cmi.2008.5 [doi]
AB  - Dendritic cells (DCs) have the potency to regulate the outcome of autoimmunity 
      through the modulation of immune responses. The induction of antigen specific 
      tolerance is critical for prevention and treatment of allograft rejection. In the 
      present study, we transfected IL-10 gene into DCs and investigated their effect 
      on inhibition of lymphocyte activity in vitro and induction of immune tolerance 
      on islet allograft in mice. An IDDM C57BL/6 mouse model was induced by 
      streptozotocin. The islet cells isolated from the BALB/c mice were transplanted 
      into the kidney capules of the model mice followed by injection of IL-10 modified 
      DCs (mDCs). The results showed that mDCs could significantly inhibit T lymphocyte 
      proliferation mediated by allotype cells and induce its apoptosis, whereas, 
      unmodified DCs (umDCs) could promote the murine lymphocyte proliferation 
      markedly. The injection of mDCs could prolong the survival of allotype islet 
      transplanted IDDM mice. The average plasma glucose (PG) level in mDCs treated 
      mice returned to normal within 3 days and lasted for about 2 weeks. The rejection 
      response in control mice occurred for 5 days after transplantation. The level of 
      IFN-gamma was lower while IL-4 was higher in mDCs treated mice than that in umDCs 
      treated mice, which indicated that Th1/Th2 deviation occurred. Our studies 
      suggest that IL-10 gene modified DCs can induce the immune tolerance to islet 
      graft and prolong survival of the recipients by the inhibiting of T cell 
      proliferation in allotype mice.
FAU - Zhu, Huifen
AU  - Zhu H
AD  - Department of Immunology, Tongji Medical College, Huazhong University of Science 
      and Technology, Wuhan 430030, China.
FAU - Qiu, Wenhong
AU  - Qiu W
FAU - Lei, Ping
AU  - Lei P
FAU - Zhou, Wei
AU  - Zhou W
FAU - Wen, Xue
AU  - Wen X
FAU - He, Fengrong
AU  - He F
FAU - Li, Li
AU  - Li L
FAU - Dai, Hong
AU  - Dai H
FAU - Shen, Guanxin
AU  - Shen G
FAU - Gong, Feili
AU  - Gong F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Cell Mol Immunol
JT  - Cellular & molecular immunology
JID - 101242872
RN  - 0 (Blood Glucose)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Blood Glucose/analysis
MH  - Cell Proliferation
MH  - Dendritic Cells/*immunology/metabolism
MH  - Diabetes Mellitus, Type 1/*immunology/metabolism/surgery
MH  - Glucose Tolerance Test
MH  - Graft Rejection
MH  - *Immune Tolerance
MH  - Interferon-gamma/biosynthesis/immunology
MH  - Interleukin-10/genetics/*immunology/metabolism
MH  - Interleukin-4/blood
MH  - Islets of Langerhans Transplantation/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Th1 Cells/*immunology/metabolism
MH  - Transfection
MH  - Transplantation Tolerance
MH  - Transplantation, Homologous
PMC - PMC4072399
EDAT- 2008/03/06 09:00
MHDA- 2008/07/17 09:00
PMCR- 2008/02/01
CRDT- 2008/03/06 09:00
PHST- 2008/03/06 09:00 [pubmed]
PHST- 2008/07/17 09:00 [medline]
PHST- 2008/03/06 09:00 [entrez]
PHST- 2008/02/01 00:00 [pmc-release]
AID - cmi20085 [pii]
AID - 10.1038/cmi.2008.5 [doi]
PST - ppublish
SO  - Cell Mol Immunol. 2008 Feb;5(1):41-6. doi: 10.1038/cmi.2008.5.