PMID- 18322732 OWN - NLM STAT- MEDLINE DCOM- 20080814 LR - 20220317 IS - 0343-8651 (Print) IS - 0343-8651 (Linking) VI - 56 IP - 6 DP - 2008 Jun TI - Effect of biocides on biofilm bacteria from dental unit water lines. PG - 619-24 LID - 10.1007/s00284-008-9136-6 [doi] AB - Microbial biofilm formation in dental unit water lines (DUWL) is a phenomenon that has been recognized for nearly four decades. Water delivered by DUWL can harbor high numbers of bacteria, including opportunistic pathogens. Biofilms on tubing within DUWL may serve as a reservoir for these microorganisms and should therefore be controlled. In this study, the effects of eight biocides were monitored on DUWL biofilms individually and in combination by epifluorescence microscopy and total viable counts (TVC). The effects of sodium dodecyl sulphate (SDS), hydrogen peroxide (H2O2), sodium hypochlorite (NaOCl), phenol (Phe), Tween 20 (Tw 20), ethylenediaminetetraacetic acid (EDTA), chlorohexidine gluconate (CHX), and povidine iodine (PI) were tested on DUWL biofilms alone and in combination. PI was found to have negligible effects on biofilm removal either applied alone or in combined form with CHX. Applying all biocides simultaneously did not completely eliminate viable bacteria nor did they remove biofilm. Overall, when combined, the biocides performed better than singly applied products. The most effective biocides were NaOCl and Phe (both alone and in combination). FAU - Liaqat, I AU - Liaqat I AD - Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore, 54590, Pakistan. iramliaq@hotmail.com FAU - Sabri, A N AU - Sabri AN LA - eng PT - Journal Article DEP - 20080306 PL - United States TA - Curr Microbiol JT - Current microbiology JID - 7808448 RN - 0 (Dental Disinfectants) RN - 0 (Disinfectants) SB - IM MH - Biofilms/*drug effects/growth & development MH - Colony Count, Microbial MH - Dental Disinfectants/*pharmacology MH - Dental Equipment/*microbiology MH - Disinfectants/*pharmacology MH - Equipment Contamination/prevention & control MH - Infection Control, Dental MH - Microbial Viability/*drug effects MH - *Water Microbiology EDAT- 2008/03/07 09:00 MHDA- 2008/08/15 09:00 CRDT- 2008/03/07 09:00 PHST- 2007/10/07 00:00 [received] PHST- 2008/01/18 00:00 [accepted] PHST- 2008/03/07 09:00 [pubmed] PHST- 2008/08/15 09:00 [medline] PHST- 2008/03/07 09:00 [entrez] AID - 10.1007/s00284-008-9136-6 [doi] PST - ppublish SO - Curr Microbiol. 2008 Jun;56(6):619-24. doi: 10.1007/s00284-008-9136-6. Epub 2008 Mar 6.