PMID- 18323745
OWN - NLM
STAT- MEDLINE
DCOM- 20081118
LR  - 20080919
IS  - 1540-0514 (Electronic)
IS  - 1073-2322 (Linking)
VI  - 30
IP  - 4
DP  - 2008 Oct
TI  - Altered gene expression patterns in dendritic cells after severe trauma: 
      implications for systemic inflammation and organ injury.
PG  - 344-51
LID - 10.1097/SHK.0b013e3181673eb4 [doi]
AB  - Dendritic cells (DCs) are professional antigen-presenting cells and members of 
      the adoptive immunity. In addition, they play an important role in innate 
      immunity within the systemic inflammatory response to trauma and sepsis. In this 
      study, gene expression patterns of DC in patients with multiple trauma were 
      studied. Total RNA was isolated from highly purified DCs (purity>95%) that were 
      enriched from peripheral blood mononuclear cells and whole blood, respectively. 
      Samples were obtained from 10 multiple trauma patients (injury severity score, 
      35.4+/-10.6 on day of admission) and 5 healthy volunteers (control). Aliquots of 
      target cDNAs and reference samples (cDNA derived from the monocytic cell line 
      SIGM5) were cohybridized on a thematic medium-density microarray assessing 780 
      inflammation-related transcripts. Twenty transcripts were up-regulated in DCs of 
      multiple trauma patients compared with healthy volunteers, whereas these 
      differences were missed when RNA from whole blood was subjected to transcriptomic 
      profiling. This cluster included central effector molecules of DC such as 
      transcripts encoding for 5-lipoxygenase and the corresponding leukotriene 4 
      receptor, which regulate DC migration, adoptive immune responses, and airway 
      inflammation, as well as CD74, CXCL4, or platelet factor 4, a chemokine not 
      implicated as a product of DCs to date. In addition, genes involved in 
      antiapoptosis (BCL2), intracellular signal transduction (mitogen-activated 
      protein kinase), and secretion of mediators (VAMP2) were found to be 
      up-regulated. The up-regulated transcripts suggest that life span and signaling 
      function of DCs are altered by trauma. Furthermore, these data confirm and expand 
      the central role of chemokines and lipid mediators as effector molecules of 
      DC-mediated immune responses in systemic inflammation associated with severe 
      trauma.
FAU - Maier, Marcus
AU  - Maier M
AD  - Department of Trauma Surgery, Hospital of the J.W. Goethe-University, Frankfurt, 
      Germany. Marcus.Maier@kgu.de
FAU - Wutzler, Sebastian
AU  - Wutzler S
FAU - Bauer, Michael
AU  - Bauer M
FAU - Trendafilov, Petar
AU  - Trendafilov P
FAU - Henrich, Dirk
AU  - Henrich D
FAU - Marzi, Ingo
AU  - Marzi I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Shock
JT  - Shock (Augusta, Ga.)
JID - 9421564
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Adult
MH  - Cell Movement
MH  - Dendritic Cells/*cytology/*metabolism
MH  - Female
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Immunosuppressive Agents/pharmacology
MH  - *Inflammation
MH  - Leukocytes, Mononuclear/cytology
MH  - Male
MH  - MicroRNAs/metabolism
MH  - Models, Biological
MH  - Oligonucleotide Array Sequence Analysis
MH  - Transcription, Genetic
EDAT- 2008/03/08 09:00
MHDA- 2008/11/19 09:00
CRDT- 2008/03/08 09:00
PHST- 2008/03/08 09:00 [pubmed]
PHST- 2008/11/19 09:00 [medline]
PHST- 2008/03/08 09:00 [entrez]
AID - 10.1097/SHK.0b013e3181673eb4 [doi]
PST - ppublish
SO  - Shock. 2008 Oct;30(4):344-51. doi: 10.1097/SHK.0b013e3181673eb4.