PMID- 1832405
OWN - NLM
STAT- MEDLINE
DCOM- 19911017
LR  - 20190912
IS  - 0435-1339 (Print)
IS  - 0435-1339 (Linking)
VI  - 26
IP  - 3
DP  - 1991 Jun
TI  - Primary culture of human gallbladder epithelial cells.
PG  - 363-9
AB  - Growth requirements of epithelial cells isolated from the human gallbladder were 
      examined. The growth of gallbladder epithelial cells (GBEC) was stimulated by 
      medium conditioned with gallbladder fibroblasts (CM-GBF) in a dose-dependent 
      manner. The conditioned medium derived from human embryo lung fibroblasts also 
      showed similar growth stimulating activity for GBEC, suggesting that fibroblasts 
      secrete a factor or factors which induce GBEC growth in vitro. CM-GBF in the 
      presence of 10% fetal bovine serum increased GBEC growth up to 10 times the 
      growth in the absence of CM-GBF supplement. GBEC cultured with CM-GBF showed 
      hexagonal shape under a phase-contrast microscope, and also expressed cytokeratin 
      and mucopolysaccharide in the cytoplasm, both of which are specific for GBEC. 
      Electron microscopy revealed desmosomes and tight junctions between the cells and 
      microvilli on the apical plasma membrane, suggesting that they regained 
      morphological polarity in the medium containing CM-GBF. These results shows that 
      CM-GBF is essential for the growth and the differentiation of GBEC in culture.
FAU - Kobayashi, K
AU  - Kobayashi K
AD  - Third Department of Internal Medicine, Tohoku University School of Medicine, 
      Sendai, Japan.
FAU - Kan, M
AU  - Kan M
FAU - Yamane, I
AU  - Yamane I
FAU - Ishii, M
AU  - Ishii M
FAU - Toyota, T
AU  - Toyota T
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Gastroenterol Jpn
JT  - Gastroenterologia Japonica
JID - 0152744
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Growth Substances)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Division/physiology
MH  - Cells, Cultured
MH  - Embryo, Mammalian/cytology
MH  - Epithelial Cells
MH  - Epithelium/metabolism/ultrastructure
MH  - Fibroblasts/cytology/metabolism
MH  - Gallbladder/*cytology/ultrastructure
MH  - Glycosaminoglycans/biosynthesis
MH  - Growth Substances/metabolism/physiology
MH  - Humans
MH  - In Vitro Techniques
MH  - Lung/cytology
MH  - Microscopy, Electron
EDAT- 1991/06/01 00:00
MHDA- 1991/06/01 00:01
CRDT- 1991/06/01 00:00
PHST- 1991/06/01 00:00 [pubmed]
PHST- 1991/06/01 00:01 [medline]
PHST- 1991/06/01 00:00 [entrez]
AID - 10.1007/BF02781926 [doi]
PST - ppublish
SO  - Gastroenterol Jpn. 1991 Jun;26(3):363-9. doi: 10.1007/BF02781926.