PMID- 18324723
OWN - NLM
STAT- MEDLINE
DCOM- 20080421
LR  - 20080312
IS  - 1615-9861 (Electronic)
IS  - 1615-9853 (Linking)
VI  - 8
IP  - 5
DP  - 2008 Mar
TI  - Proteomic profiling reveals that Th2-inducing dendritic cells stimulated with 
      helminth antigens have a 'limited maturation' phenotype.
PG  - 980-93
LID - 10.1002/pmic.200700538 [doi]
AB  - Dendritic cells (DCs) are important in the initiation of primary immune responses 
      against pathogens. To aid understanding of how DCs guide T helper (Th)2-type 
      responses, we employed 2-DE in association with MS/MS to identify proteins which 
      characterise pro-Th2 DCs (matured with zero-to-three hours released proteins 
      (0-3hRP), released by Schistosoma mansoni cercariae) versus pro-Th1 DCs (matured 
      with lipopolysaccharide, LPS) and immature DCs. Software analysis of average 2-DE 
      gels (three replicates per DC type) showed many similarities in the pattern of 
      spots between the three groups of DCs but also marked changes. The major and 
      significant changes in protein expression mainly affected cytoskeletal proteins. 
      Other differences included chaperone proteins and enzymes involved in protein 
      folding, S100 calcium-binding proteins, peroxiredoxin 1, superoxide dismutase 1, 
      several annexins and arginase 1. Our study demonstrates that pro-Th2 DCs matured 
      with 0-3hRP exhibit a proteome that is intermediate between that of immature DCs 
      and pro-Th1 DCs. Finally, the differential regulation of protein spots identified 
      by MALDI-MS/MS as having cytoskeletal and morphological functions was confirmed 
      by contrast, confocal and scanning electron microscopy examination of DCs. 
      Together, our results support the view that Th2 differentiation results from a 
      'limited maturation' of DCs.
FAU - Ferret-Bernard, Stephanie
AU  - Ferret-Bernard S
AD  - Department of Biology (Area 5), University of York, York, UK.
FAU - Curwen, Rachel Sally
AU  - Curwen RS
FAU - Mountford, Adrian Paul
AU  - Mountford AP
LA  - eng
GR  - 068509/Wellcome Trust/United Kingdom
GR  - 071762/Wellcome Trust/United Kingdom
GR  - 072255/Wellcome Trust/United Kingdom
GR  - BBS/B/08531/Biotechnology and Biological Sciences Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (Antigens, Helminth)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Proteome)
SB  - IM
MH  - Animals
MH  - Antigens, Helminth/*immunology
MH  - Dendritic Cells/*chemistry/immunology
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Female
MH  - Lipopolysaccharides/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Proteome/*analysis
MH  - Schistosoma mansoni/*immunology
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Th2 Cells/*immunology
EDAT- 2008/03/08 09:00
MHDA- 2008/04/22 09:00
CRDT- 2008/03/08 09:00
PHST- 2008/03/08 09:00 [pubmed]
PHST- 2008/04/22 09:00 [medline]
PHST- 2008/03/08 09:00 [entrez]
AID - 10.1002/pmic.200700538 [doi]
PST - ppublish
SO  - Proteomics. 2008 Mar;8(5):980-93. doi: 10.1002/pmic.200700538.