PMID- 18325077
OWN - NLM
STAT- MEDLINE
DCOM- 20080722
LR  - 20130118
IS  - 1398-9995 (Electronic)
IS  - 0105-4538 (Linking)
VI  - 63
IP  - 6
DP  - 2008 Jun
TI  - A high-affinity monoclonal anti-IgE antibody for depletion of IgE and IgE-bearing 
      cells.
PG  - 695-702
LID - 10.1111/j.1398-9995.2008.01664.x [doi]
AB  - BACKGROUND: We have identified a monoclonal anti-human immunoglobulin E (IgE) 
      antibody, which recognizes FcepsilonRI-bound IgE and prevents binding of IgE to 
      FcepsilonRI. In this study, we assessed the binding kinetics and affinity of 
      monoclonal antibody 12 (mAb12) for IgE and investigated whether mAb12 can be used 
      for depletion of IgE and isolation of IgE-bearing cells from peripheral blood. 
      METHODS: Binding kinetics and affinity for IgE were studied using Biacore surface 
      plasmon resonance technique experiments. IgE antibodies were depleted from serum 
      using sepharose-coupled mAb12 and IgE-bearing cells were enriched from 
      heparinized blood samples with mAb12. The extent and biological relevance of IgE 
      depletion were studied by quantitative IgE measurements and basophil histamine 
      release experiments. Specific binding of mAb12 to IgE-bearing cells (basophils, 
      mast cells, IgE-secreting plasma cells) was demonstrated by FACS. RESULTS: 
      Monoclonal antibody 12 shows rapid association (k(a) = 5.46e5/Ms) with IgE, 
      almost no dissociation (k(d) = 8.8e-5/s) and an affinity for IgE (K(D) = 1.61e-10 
      M), which is as high as that of FcepsilonRI. Immobilized mAb12 could be used to 
      deplete IgE antibodies and isolate IgE-bearing cells from peripheral blood in a 
      single-step procedure. CONCLUSIONS: Monoclonal antibody 12 is a high affinity 
      anti-human IgE antibody, which efficiently removes IgE and IgE-bearing cells from 
      peripheral blood and may thus be used for extracorporeal depletion of IgE and 
      IgE-bearing cells.
FAU - Laffer, S
AU  - Laffer S
AD  - Division of Immunopathology, Department of Pathophysiology, Center for Physiology 
      and Pathophysiology, Medical University of Vienna, Vienna, Austria.
FAU - Lupinek, C
AU  - Lupinek C
FAU - Rauter, I
AU  - Rauter I
FAU - Kneidinger, M
AU  - Kneidinger M
FAU - Drescher, A
AU  - Drescher A
FAU - Jordan, J-H
AU  - Jordan JH
FAU - Krauth, M-T
AU  - Krauth MT
FAU - Valent, P
AU  - Valent P
FAU - Kricek, F
AU  - Kricek F
FAU - Spitzauer, S
AU  - Spitzauer S
FAU - Englund, H
AU  - Englund H
FAU - Valenta, R
AU  - Valenta R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080305
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Allergens)
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (FCER1A protein, human)
RN  - 0 (Receptors, IgE)
RN  - 0 (anti-IgE antibodies)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adult
MH  - Allergens/immunology
MH  - Antibodies, Anti-Idiotypic/*immunology
MH  - Antibodies, Monoclonal/*immunology/metabolism
MH  - *Antibody Affinity
MH  - Basophils/immunology/metabolism
MH  - Female
MH  - Histamine Release
MH  - Humans
MH  - Immunoglobulin E/blood/*immunology
MH  - Male
MH  - Mast Cells/immunology/metabolism
MH  - Middle Aged
MH  - Plasma Cells/immunology/metabolism
MH  - Pollen/immunology
MH  - Receptors, IgE/*immunology/metabolism
MH  - Rhinitis, Allergic, Seasonal/*immunology
EDAT- 2008/03/08 09:00
MHDA- 2008/07/23 09:00
CRDT- 2008/03/08 09:00
PHST- 2008/03/08 09:00 [pubmed]
PHST- 2008/07/23 09:00 [medline]
PHST- 2008/03/08 09:00 [entrez]
AID - ALL1664 [pii]
AID - 10.1111/j.1398-9995.2008.01664.x [doi]
PST - ppublish
SO  - Allergy. 2008 Jun;63(6):695-702. doi: 10.1111/j.1398-9995.2008.01664.x. Epub 2008 
      Mar 5.