PMID- 18325722
OWN - NLM
STAT- MEDLINE
DCOM- 20080815
LR  - 20181201
IS  - 0739-7240 (Print)
IS  - 0739-7240 (Linking)
VI  - 35
IP  - 1
DP  - 2008 Jul
TI  - Patterns of gene expression in pig adipose tissue: insulin-like growth factor 
      system proteins, neuropeptide Y (NPY), NPY receptors, neurotrophic factors and 
      other secreted factors.
PG  - 24-34
LID - 10.1016/j.domaniend.2008.01.004 [doi]
AB  - Although cDNA microarray studies have examined gene expression in human and 
      rodent adipose tissue, only one microarray study of adipose tissue from growing 
      pigs has been reported. Total RNA was collected at slaughter from outer 
      subcutaneous adipose tissue (OSQ) and middle subcutaneous adipose tissue (MSQ) 
      from gilts at 90, 150, and 210 d (n=5 age(-1)). Dye labeled cDNA probes were 
      hybridized to custom porcine microarrays (70-mer oligonucleotides). Gene 
      expression of insulin-like growth factor binding proteins (IGFBPs), hormones, 
      growth factors, neuropeptide Y (NPY) receptors (NPYRs) and other receptors in OSQ 
      and MSQ changed little with age in growing pigs. Distinct patterns of relative 
      gene expression were evident within NPYR and IGFBP family members in adipose 
      tissue from growing pigs. Relative gene expression levels of NPY2R, NPY4R and 
      angiopoietin 2 (ANG-2) distinguished OSQ and MSQ depots in growing pigs. We 
      demonstrated, for the first time, the expression of IGFBP-7, IGFBP-5, NPY1R, 
      NPY2R, NPY, connective tissue growth factor (CTGF), brain-derived neurotrophic 
      factor (BDNF) and ciliary neurotrophic factor (CNTF) genes in pig adipose tissue 
      with microarray and RT-PCR assays. Furthermore, adipose tissue CTGF gene 
      expression was upregulated while NPY and NPY2R gene expression were significantly 
      down regulated by age. These studies demonstrate that expression of neuropeptides 
      and neurotrophic factors in pig adipose tissue may be involved in regulation of 
      leptin secretion. Many other regulatory factors were not influenced by age in 
      growing pigs but may be influenced by location or depot.
FAU - Hausman, G J
AU  - Hausman GJ
AD  - United States Department of Agriculture, Agricultural Research Service, Richard 
      B. Russell Agricultural Research Center, 950 College Station Road, Athens, GA 
      30605, USA. Gary.Hausman@ars.usda.gov
FAU - Barb, C R
AU  - Barb CR
FAU - Dean, R G
AU  - Dean RG
LA  - eng
PT  - Journal Article
DEP - 20080212
PL  - United States
TA  - Domest Anim Endocrinol
JT  - Domestic animal endocrinology
JID - 8505191
RN  - 0 (Insulin-Like Growth Factor Binding Proteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neuropeptide Y)
RN  - 0 (Receptors, Neuropeptide Y)
RN  - 0 (Somatomedins)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Gene Expression Profiling
MH  - Growth and Development/genetics
MH  - Insulin-Like Growth Factor Binding Proteins/genetics/metabolism
MH  - Intercellular Signaling Peptides and Proteins/*genetics/metabolism
MH  - Nerve Growth Factors/*genetics/metabolism
MH  - Neuropeptide Y/*genetics/metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - Receptors, Neuropeptide Y/*genetics/metabolism
MH  - Somatomedins/genetics/metabolism
MH  - Subcutaneous Fat/*metabolism
MH  - Swine/*genetics/growth & development/metabolism
EDAT- 2008/03/08 09:00
MHDA- 2008/08/16 09:00
CRDT- 2008/03/08 09:00
PHST- 2007/09/17 00:00 [received]
PHST- 2008/01/08 00:00 [revised]
PHST- 2008/01/09 00:00 [accepted]
PHST- 2008/03/08 09:00 [pubmed]
PHST- 2008/08/16 09:00 [medline]
PHST- 2008/03/08 09:00 [entrez]
AID - S0739-7240(08)00007-6 [pii]
AID - 10.1016/j.domaniend.2008.01.004 [doi]
PST - ppublish
SO  - Domest Anim Endocrinol. 2008 Jul;35(1):24-34. doi: 
      10.1016/j.domaniend.2008.01.004. Epub 2008 Feb 12.