PMID- 18326537
OWN - NLM
STAT- MEDLINE
DCOM- 20080530
LR  - 20131121
IS  - 1462-0332 (Electronic)
IS  - 1462-0324 (Linking)
VI  - 47
IP  - 5
DP  - 2008 May
TI  - Prevention of corticosteroid-induced osteonecrosis in rabbits by intra-bone 
      marrow injection of autologous bone marrow cells.
PG  - 591-6
LID - 10.1093/rheumatology/ken037 [doi]
AB  - OBJECTIVES: Femoral head osteonecrosis (ON) is a serious complication of steroid 
      administration. We evaluated bone marrow transplantation (BMT) for preventing 
      corticosteroid-induced ON. METHODS: Rabbits, injected with methylprednisolone 
      (MPSL; 20 mg/kg), were divided into four groups: (i) MPSL alone; MPSL injection 
      only, (ii) MPSL+needling; 2 days after MPSL injection, a hole (1.2 mm diameter) 
      was drilled from the outer cortex 2.5 cm distal to the proximal end of the 
      greater trochanter, (iii) MPSL+saline; 2 days after MPSL injection, 2 ml saline 
      was injected directly into the bone marrow cavity, and (iv) MPSL+BMT; 2 days 
      after MPSL injection, 1 x 10(7)/2 ml bone marrow cells (BMCs) were injected 
      directly into the bone marrow cavity. Platelets, fibrinogen, prothrombin time and 
      total cholesterol in peripheral blood were measured before and after treatment. 
      Tissues were stained with haematoxylin and eosion and terminal 
      deoxynucleotidyl-mediated deoxyuridine triphosphate nick-end labelling stain and 
      immunostained for VEGF, while cell proliferation and viability of whole BMCs in 
      the femur were analysed by cell cycle analysis and [(3)H]-thymidine uptake. 
      RESULTS: The ON incidence in rabbits treated with MPSL alone, MPSL+needling and 
      MPSL+saline was 72.7, 70.0 and 66.7%, respectively, while in the MPSL+BMT group, 
      the incidence was 0%. Serological findings in the MPSL+BMT group were almost 
      normalized. VEGF and TUNEL staining were reduced in the MPSL+BMT group compared 
      with all other groups. There were significantly fewer BMCs in G1 phase from the 
      MPSL+BMT group than the other groups, while uptake of [(3)H]-thymidine was 
      significantly increased. CONCLUSION: Direct injection of autologous BMCs into 
      femurs prevents corticosteroid-induced ON following treatment with high-dose, 
      short-term steroids.
FAU - Asada, T
AU  - Asada T
AD  - Department of Orthopaedic Surgery, Kansai Medical University, 10-15 Fumizono-cho, 
      Moriguchi City, Osaka 570-8506, Japan.
FAU - Kushida, T
AU  - Kushida T
FAU - Umeda, M
AU  - Umeda M
FAU - Oe, K
AU  - Oe K
FAU - Matsuya, H
AU  - Matsuya H
FAU - Wada, T
AU  - Wada T
FAU - Sasai, K
AU  - Sasai K
FAU - Ikehara, S
AU  - Ikehara S
FAU - Iida, H
AU  - Iida H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080307
PL  - England
TA  - Rheumatology (Oxford)
JT  - Rheumatology (Oxford, England)
JID - 100883501
RN  - 0 (Glucocorticoids)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - X4W7ZR7023 (Methylprednisolone)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Blood Coagulation
MH  - Bone Marrow Transplantation/*methods
MH  - Cell Cycle/drug effects
MH  - Drug Administration Schedule
MH  - Female
MH  - Femur Head/pathology
MH  - Femur Head Necrosis/*chemically induced/pathology/*prevention & control
MH  - Fibrinolysis
MH  - Glucocorticoids/*adverse effects
MH  - In Situ Nick-End Labeling
MH  - Injections
MH  - Methylprednisolone/*adverse effects
MH  - Models, Animal
MH  - Osteoblasts/transplantation
MH  - Osteoclasts/transplantation
MH  - Rabbits
MH  - Transplantation, Autologous
MH  - Vascular Endothelial Growth Factor A/analysis
EDAT- 2008/03/11 09:00
MHDA- 2008/05/31 09:00
CRDT- 2008/03/11 09:00
PHST- 2008/03/11 09:00 [pubmed]
PHST- 2008/05/31 09:00 [medline]
PHST- 2008/03/11 09:00 [entrez]
AID - ken037 [pii]
AID - 10.1093/rheumatology/ken037 [doi]
PST - ppublish
SO  - Rheumatology (Oxford). 2008 May;47(5):591-6. doi: 10.1093/rheumatology/ken037. 
      Epub 2008 Mar 7.