PMID- 18326759
OWN - NLM
STAT- MEDLINE
DCOM- 20080415
LR  - 20091119
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 49
IP  - 3
DP  - 2008 Mar
TI  - BDNF increases survival of retinal dopaminergic neurons after prenatal 
      compromise.
PG  - 1282-9
LID - 10.1167/iovs.07-0521 [doi]
AB  - PURPOSE: Chronic placental insufficiency (CPI) severe enough to cause growth 
      restriction (GR) results in alterations to the retina, including a reduction in 
      tyrosine hydroxylase immunoreactive (TH-IR)-dopaminergic amacrine cells. 
      Brain-derived neurotrophic factor (BDNF) plays a role in the development of the 
      retinal dopaminergic network and may therefore be an appropriate therapy for 
      restoring dopaminergic cells after prenatal compromise. This study was conducted 
      (1) to establish whether BDNF and its receptor NTRK2 (Trk B) are altered in the 
      retina after CPI and (2) to explore the potential of BDNF to enhance dopaminergic 
      cell survival in organotypic retinal cultures from prenatally compromised 
      animals. METHODS: CPI was induced in pregnant guinea pigs at 30 days' gestation 
      (dg; term, approximately 67 dg) via unilateral ligation of the uterine artery. 
      Fetuses were euthanatized at 60 dg and the retinas prepared for enzyme-linked 
      immunosorbent assay (ELISA) analysis of BDNF protein levels and for 
      immunohistochemistry to localize BDNF and NTRK2. Organotypic cultures of retinas 
      from GR and control fetuses at 50 to 52 dg were treated with BDNF, and 
      dopaminergic amacrine cells counts were assessed. RESULTS: Retinal BDNF protein 
      levels and the intensity of BDNF-immunoreactivity (IR) in the ganglion cell layer 
      were reduced (P < 0.05) in GR fetuses compared with control fetuses. Addition of 
      BDNF to organotypic cultures increased (P < 0.05) the survival and neurite growth 
      of dopaminergic neurons from both control and GR fetuses. CONCLUSIONS: 
      Alterations to BDNF levels may underlie reductions in dopaminergic amacrine cells 
      observed after CPI. The addition of BDNF has the potential to increase survival 
      and neurite growth of dopaminergic amacrine cells.
FAU - Loeliger, Michelle M
AU  - Loeliger MM
AD  - Department of Anatomy and Cell Biology, University of Melbourne, Melbourne, 
      Victoria, Australia. m.loeliger@unimelb.edu.au
FAU - Briscoe, Todd
AU  - Briscoe T
FAU - Rees, Sandra M
AU  - Rees SM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Amacrine Cells/cytology/*metabolism
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism/pharmacology
MH  - Cell Count
MH  - Cell Survival/drug effects/physiology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Fetal Growth Retardation/*metabolism
MH  - Guinea Pigs
MH  - Immunoenzyme Techniques
MH  - Organ Culture Techniques
MH  - Placental Insufficiency/*metabolism
MH  - Pregnancy
MH  - Receptor, trkB/*metabolism
MH  - Retina/drug effects/*metabolism
MH  - Retinal Ganglion Cells/metabolism
MH  - Tyrosine 3-Monooxygenase/*metabolism
EDAT- 2008/03/11 09:00
MHDA- 2008/04/16 09:00
CRDT- 2008/03/11 09:00
PHST- 2008/03/11 09:00 [pubmed]
PHST- 2008/04/16 09:00 [medline]
PHST- 2008/03/11 09:00 [entrez]
AID - 49/3/1282 [pii]
AID - 10.1167/iovs.07-0521 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2008 Mar;49(3):1282-9. doi: 10.1167/iovs.07-0521.