PMID- 18327208
OWN - NLM
STAT- MEDLINE
DCOM- 20080812
LR  - 20230210
IS  - 0893-3952 (Print)
IS  - 0893-3952 (Linking)
VI  - 21
IP  - 6
DP  - 2008 Jun
TI  - Diagnostic importance of 9p21 homozygous deletion in malignant mesotheliomas.
PG  - 742-7
LID - 10.1038/modpathol.2008.45 [doi]
AB  - Definitive diagnosis of malignant mesothelioma in small specimens can be 
      extremely difficult based on morphology alone. Homozygous deletion of 9p21, the 
      locus harboring the p16 gene, has been reported as the most common genetic 
      alteration in malignant mesotheliomas. Recent studies demonstrated that this 
      alteration may be useful for differentiating benign from malignant mesothelial 
      proliferations in cytology specimens. The aim of this study was to evaluate the 
      diagnostic utility of homozygous deletion of 9p21 assessed by fluorescence in 
      situ hybridization (FISH) in mesothelial proliferations involving serosal 
      surfaces in paraffin-embedded tissue. p16 protein immunoexpression was also 
      explored as a potential diagnostic aid. FISH analysis demonstrated homozygous 
      deletion of the 9p21 locus in 35 of 52 cases (67%) of pleural mesothelioma and in 
      5 of 20 cases of peritoneal mesothelioma (25%) (P<0.005). None of 40 cases of 
      reactive pleural mesothelial proliferations showed p16 deletion (P<0.005). Loss 
      of immunoexpression of p16 was observed in 71% of the peritoneal mesotheliomas, 
      40% of the pleural malignant mesotheliomas and 15% of the reactive mesothelial 
      cells. Homozygous deletion did not correlate with p16 protein expression in any 
      of the studied groups. Our study suggests that 9p21 homozygous deletion assessed 
      by FISH on paraffin-embedded tissue may be helpful for differentiating between 
      malignant mesotheliomas and reactive mesothelial proliferations. A discrepancy 
      between p16 protein expression and homozygous deletion suggests that other 
      molecular mechanisms may play a role in p16 protein expression in mesothelial 
      proliferations.
FAU - Chiosea, Simion
AU  - Chiosea S
AD  - Division of Anatomic Pathology, Department of Pathology, University of Pittsburgh 
      Medical Center, Presbyterian University Hospital, Pittsburgh, PA 15213, USA.
FAU - Krasinskas, Alyssa
AU  - Krasinskas A
FAU - Cagle, Philip T
AU  - Cagle PT
FAU - Mitchell, Kisha A
AU  - Mitchell KA
FAU - Zander, Dani S
AU  - Zander DS
FAU - Dacic, Sanja
AU  - Dacic S
LA  - eng
PT  - Journal Article
DEP - 20080307
PL  - United States
TA  - Mod Pathol
JT  - Modern pathology : an official journal of the United States and Canadian Academy 
      of Pathology, Inc
JID - 8806605
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Biomarkers, Tumor/analysis
MH  - *Gene Deletion
MH  - *Genes, p16
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Mesothelioma/diagnosis/*genetics
MH  - Peritoneal Neoplasms/diagnosis/*genetics
MH  - Pleural Neoplasms/diagnosis/*genetics
EDAT- 2008/03/11 09:00
MHDA- 2008/08/13 09:00
CRDT- 2008/03/11 09:00
PHST- 2008/03/11 09:00 [pubmed]
PHST- 2008/08/13 09:00 [medline]
PHST- 2008/03/11 09:00 [entrez]
AID - S0893-3952(22)02303-1 [pii]
AID - 10.1038/modpathol.2008.45 [doi]
PST - ppublish
SO  - Mod Pathol. 2008 Jun;21(6):742-7. doi: 10.1038/modpathol.2008.45. Epub 2008 Mar 
      7.