PMID- 18329891
OWN - NLM
STAT- MEDLINE
DCOM- 20080422
LR  - 20081121
IS  - 1096-0279 (Electronic)
IS  - 1046-5928 (Linking)
VI  - 59
IP  - 1
DP  - 2008 May
TI  - Periplasmic expression and recovery of human interferon gamma in Escherichia 
      coli.
PG  - 169-74
LID - 10.1016/j.pep.2008.01.019 [doi]
AB  - A synthetic human interferon gamma (hIFN-gamma) gene was fused to SP1 and SP3, 
      two Sec-dependent artificial signal peptides to transport the hIFN-gamma to the 
      periplasm of Escherichia coli BL21-SI. The processing efficiency of both 
      SP1-hIFN-gamma and SP3-hIFN-gamma was dependent on the culture medium as well as 
      the post-induction temperature. Both precursors were processed completely when 
      cells were cultured using minimal medium and a post-induction temperature of 32.5 
      degrees C, and only the processed hIFN-gamma was detected. The SP3 signal peptide 
      was more efficient than SP1 for the secretion of hIFN-gamma. Sixty percent of the 
      total hIFN-gamma was secreted to the periplasm using the SP3 signal peptide and a 
      post-induction temperature of 20 degrees C. Using Tris-sucrose-dithiothreitol 
      (TSD) hypertonic buffer, the periplasmic soluble hINF-gamma was recovered with a 
      purity of 85%.
FAU - Balderas Hernandez, Victor E
AU  - Balderas Hernandez VE
AD  - Division de Biologia Molecular, Instituto Potosino de Investigacion Cientifica y 
      Tecnologica, Camino a la Presa San Jose 2055, Col. Lomas 4a. Seccion, C.P. 78216 
      San Luis Potosi, SLP, Mexico.
FAU - Paz Maldonado, Luz M T
AU  - Paz Maldonado LM
FAU - Medina Rivero, Emilio
AU  - Medina Rivero E
FAU - Barba de la Rosa, Ana P
AU  - Barba de la Rosa AP
FAU - Jimenez-Bremont, Juan F
AU  - Jimenez-Bremont JF
FAU - Ordonez Acevedo, Leandro G
AU  - Ordonez Acevedo LG
FAU - De Leon Rodriguez, Antonio
AU  - De Leon Rodriguez A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080205
PL  - United States
TA  - Protein Expr Purif
JT  - Protein expression and purification
JID - 9101496
RN  - 0 (Protein Sorting Signals)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Escherichia coli/*metabolism/ultrastructure
MH  - Humans
MH  - Interferon-gamma/*biosynthesis/isolation & purification
MH  - Molecular Sequence Data
MH  - Periplasm/*metabolism
MH  - Protein Sorting Signals/genetics
MH  - Temperature
EDAT- 2008/03/11 09:00
MHDA- 2008/04/23 09:00
CRDT- 2008/03/11 09:00
PHST- 2007/11/29 00:00 [received]
PHST- 2008/01/22 00:00 [revised]
PHST- 2008/01/23 00:00 [accepted]
PHST- 2008/03/11 09:00 [pubmed]
PHST- 2008/04/23 09:00 [medline]
PHST- 2008/03/11 09:00 [entrez]
AID - S1046-5928(08)00031-4 [pii]
AID - 10.1016/j.pep.2008.01.019 [doi]
PST - ppublish
SO  - Protein Expr Purif. 2008 May;59(1):169-74. doi: 10.1016/j.pep.2008.01.019. Epub 
      2008 Feb 5.