PMID- 18330944
OWN - NLM
STAT- MEDLINE
DCOM- 20080730
LR  - 20211020
IS  - 1007-9327 (Print)
IS  - 1007-9327 (Linking)
VI  - 14
IP  - 10
DP  - 2008 Mar 14
TI  - Gastric juice for the diagnosis of H pylori infection in patients on proton pump 
      inhibitors.
PG  - 1539-43
AB  - AIM: To determine the efficacy of gastric juice polymerase chain reaction (PCR) 
      for the detection of H pylori infection in comparison with histology and gastric 
      antral biopsy PCR in patients on a proton pump inhibitor (PPI). METHODS: 
      Eighty-five consecutive patients with dyspeptic symptoms were enrolled. Gastric 
      biopsies for histology, PCR and gastric juice were collected at endoscopy for PCR 
      of the H pylori urease C gene (ure C). Sensitivity, specificity, positive 
      predictive value (PPV), negative predictive value (NPV), accuracy, positive and 
      negative likelihood ratio for PCR of gastric juice for the H pylori ure C gene 
      was compared to histology and gastric antral biopsy H pylori ure C PCR in 
      patients with and without PPI. RESULTS: Gastric juice PCR was positive in 66 
      (78%) patients. Histology showed H pylori associated gastritis in 57 (67%). 
      Gastric biopsy PCR was positive in 72 (85%). In patients not taking PPI, the 
      sensitivity, specificity, PPV, NPV, accuracy and positive and negative likelihood 
      ratio for gastric juice PCR were 89%, 72%, 91%, 67%, 90%, 85%, 3.1 and 0.1 
      respectively. In patients on PPI these values were 86%, 100%%, 100%, 29%, 86%, 
      9.5 and 1.4, respectively. CONCLUSION: Gastric juice PCR for the diagnosis of H 
      pylori infection has increased sensitivity compared to histology with PPI. The 
      use of gastric juice PCR is recommended to confirm H pylori status in patients 
      taking PPIs.
FAU - Yakoob, Javed
AU  - Yakoob J
AD  - Section of Gastroenterology, Department of Medicine, Aga Khan University 
      Hospital, Stadium Road, Karachi 74800, Pakistan. yakoobjaved@hotmail.com
FAU - Rasool, Shahid
AU  - Rasool S
FAU - Abbas, Zaigham
AU  - Abbas Z
FAU - Jafri, Wasim
AU  - Jafri W
FAU - Abid, Shahab
AU  - Abid S
FAU - Islam, Muhammad
AU  - Islam M
FAU - Ahmad, Zubair
AU  - Ahmad Z
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (DNA, Bacterial)
RN  - 0 (Proton Pump Inhibitors)
RN  - EC 3.5.1.5 (Urease)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Biopsy
MH  - DNA, Bacterial/metabolism
MH  - Female
MH  - Gastric Juice/*enzymology/*microbiology
MH  - Helicobacter Infections/*diagnosis/*drug therapy/enzymology
MH  - Helicobacter pylori/enzymology/genetics/*pathogenicity
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Predictive Value of Tests
MH  - Proton Pump Inhibitors/*therapeutic use
MH  - Pyloric Antrum/enzymology/microbiology/pathology
MH  - Sensitivity and Specificity
MH  - Urease/*genetics/metabolism
PMC - PMC2693748
EDAT- 2008/03/12 09:00
MHDA- 2008/07/31 09:00
PMCR- 2008/03/14
CRDT- 2008/03/12 09:00
PHST- 2008/03/12 09:00 [pubmed]
PHST- 2008/07/31 09:00 [medline]
PHST- 2008/03/12 09:00 [entrez]
PHST- 2008/03/14 00:00 [pmc-release]
AID - 10.3748/wjg.14.1539 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2008 Mar 14;14(10):1539-43. doi: 10.3748/wjg.14.1539.