PMID- 18331334
OWN - NLM
STAT- MEDLINE
DCOM- 20080613
LR  - 20220321
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Linking)
VI  - 10
IP  - 6
DP  - 2008 Jun
TI  - Fluorescence in situ hybridization (FISH) analysis of the interactions between 
      honeybee larvae and Paenibacillus larvae, the causative agent of American 
      foulbrood of honeybees (Apis mellifera).
PG  - 1612-20
LID - 10.1111/j.1462-2920.2008.01579.x [doi]
AB  - American foulbrood (AFB) is a bacterial disease of honeybee larvae caused by the 
      spore-forming bacterium Paenibacillus larvae. Although AFB and its aetiological 
      agent are described now for more than a century, the general and molecular 
      pathogenesis of this notifiable disease is poorly understood. We used 
      fluorescence in situ hybridization (FISH) performed with P. larvae-specific, 16S 
      rRNA-targeted oligonucleotide probes to analyse the early steps in the 
      pathogenesis of American foulbrood. The following chain of events could be 
      demonstrated: (i) the spores germinate in the midgut lumen, (ii) the vegetative 
      bacteria massively proliferate within the midgut before, and (iii) they start to 
      locally breach the epithelium and invade the haemocoel. The paracellular route 
      was shown to be the main mechanism for invasion contrasting earlier hypotheses of 
      phagocytosis of P. larvae. Invasion coincided with the death of the host 
      implicating that the penetration of the midgut epithelium is a critical step 
      determining the time of death.
FAU - Yue, Dominique
AU  - Yue D
AD  - Institute for Bee Research, Friedrich-Engels-Str. 32, 16540 Hohen Neuendorf, 
      Germany.
FAU - Nordhoff, Marcel
AU  - Nordhoff M
FAU - Wieler, Lothar H
AU  - Wieler LH
FAU - Genersch, Elke
AU  - Genersch E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080304
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bees/*microbiology
MH  - DNA, Bacterial/genetics
MH  - DNA, Ribosomal/genetics
MH  - Epithelium/microbiology
MH  - Gastrointestinal Tract/microbiology
MH  - Gram-Positive Bacteria/*growth & development/pathogenicity
MH  - *Host-Pathogen Interactions
MH  - In Situ Hybridization, Fluorescence
MH  - Larva/*microbiology
MH  - Oligonucleotide Probes/genetics
MH  - RNA, Ribosomal, 16S/genetics
EDAT- 2008/03/12 09:00
MHDA- 2008/06/14 09:00
CRDT- 2008/03/12 09:00
PHST- 2008/03/12 09:00 [pubmed]
PHST- 2008/06/14 09:00 [medline]
PHST- 2008/03/12 09:00 [entrez]
AID - EMI1579 [pii]
AID - 10.1111/j.1462-2920.2008.01579.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2008 Jun;10(6):1612-20. doi: 10.1111/j.1462-2920.2008.01579.x. 
      Epub 2008 Mar 4.