PMID- 18332005
OWN - NLM
STAT- MEDLINE
DCOM- 20090317
LR  - 20190923
IS  - 0253-9772 (Print)
IS  - 0253-9772 (Linking)
VI  - 30
IP  - 3
DP  - 2008 Mar
TI  - [Cloning of gene fragment of estrogen receptor-beta and its expression in mouse 
      embryo].
PG  - 347-51
AB  - In order to study the expression and regulation effects of estrogen receptor-beta 
      (ERbeta) in the development of mouse embryo, the primer of ERbeta was designed, 
      the ERbeta fragment was first obtained by RT-PCR and subcloned into plasmids 
      pGEM- 3Z, then the recombinant plasmids were linearized with the restriction 
      enzymes of EcoRand Hind. Using Sp6 and T7 RNA polymerase, the digoxigenin(dig) 
      labeled sense and anti-sense probes were transcriped in vitro, respectively. Then 
      the expression of ERbeta in mouse embryo was examined with the probes by 
      whole-mount in situ hybridization. The results indicated that ERbeta is expressed 
      in the brain, spinal neural tube, genital ridge, pericardium, limb bud and 
      mandibular arch of 10.5 dpc embryo, and is also expressed in the telencephalon, 
      mesencephalon, medulla oblongata, spinal cord and limb bud of 13.5 dpc embryo. 
      These results suggest that ERbeta maybe play a role of regulation in sexual 
      differentiation, primal differentiation of neural tube, further differentiation 
      of three primary cerebral vesicles and spinal cord, generation and 
      differentiation of bone and cartilage of limb bud, development of pericardium and 
      configuration differentiation of mandibular in mouse embryo.
FAU - Zhang, Zi-Feng
AU  - Zhang ZF
AD  - School of Life Science, Xuzhou Normal University, Xuzhou 221116, China. 
      zhangzifengsuper@xznu.edu.cn
FAU - Fan, Shao-Hua
AU  - Fan SH
FAU - Lu, Jun
AU  - Lu J
FAU - Wu, Dong-Mei
AU  - Wu DM
FAU - Shan, Qun
AU  - Shan Q
FAU - Hu, Bin
AU  - Hu B
FAU - Li, Fei
AU  - Li F
FAU - Zheng, Yuan-Lin
AU  - Zheng YL
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Yi Chuan
JT  - Yi chuan = Hereditas
JID - 9436478
RN  - 0 (Estrogen Receptor beta)
SB  - IM
MH  - Animals
MH  - Cloning, Molecular/*methods
MH  - Embryo, Mammalian/*metabolism
MH  - Estrogen Receptor beta/*genetics/physiology
MH  - Female
MH  - In Situ Hybridization
MH  - Mice
MH  - Plasmids/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2008/03/12 09:00
MHDA- 2009/03/18 09:00
CRDT- 2008/03/12 09:00
PHST- 2008/03/12 09:00 [pubmed]
PHST- 2009/03/18 09:00 [medline]
PHST- 2008/03/12 09:00 [entrez]
AID - 0253-9772(2008)03-347-05 [pii]
AID - 10.3724/sp.j.1005.2008.00347 [doi]
PST - ppublish
SO  - Yi Chuan. 2008 Mar;30(3):347-51. doi: 10.3724/sp.j.1005.2008.00347.