PMID- 18332537
OWN - NLM
STAT- MEDLINE
DCOM- 20080804
LR  - 20190827
IS  - 0910-6340 (Print)
IS  - 0910-6340 (Linking)
VI  - 24
IP  - 3
DP  - 2008 Mar
TI  - Poly(methylmethacrylate) microchip electrophoresis of proteins using 
      linear-poly(acrylamide) solutions as separation matrix.
PG  - 321-5
AB  - Poly(methylmethacrylate) (PMMA) microchip electrophoresis of sodium dodecyl 
      sulfate-protein complexes (SDS-PC) using linear-poly(acrylamide) (L-PA) as a 
      separation matrix was investigated. Prior to electrophoresis, channel walls of 
      PMMA were modified with methylcellulose (MC) to prevent adsorption between 
      channel walls and SDS-PC. Size-based protein separation (SBPS) was successfully 
      performed using the MC-coated microchips with Ferguson plot-fittings. The 
      entangled L-PA solution provided high resolution of peaks of SDS-PC when the 
      concentration of L-PA was increased. Some investigations into the separation 
      mechanism, such as the plot of the logarithm of mobility of each SDS-PC versus 
      the logarithm of the molecular weight of the complex exhibiting linear behavior, 
      indicated that the separation mechanism was dependent on mass discrimination, in 
      accordance with Ogston model.
FAU - Okada, Hiroki
AU  - Okada H
AD  - Department of Applied Chemistry, Graduate School of Engineering, Nagoya 
      University, Chikusa, Nagoya, Japan. h051102d@mbox.nagoya-u.ac.jp
FAU - Kaji, Noritada
AU  - Kaji N
FAU - Tokeshi, Manabu
AU  - Tokeshi M
FAU - Baba, Yoshinobu
AU  - Baba Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Anal Sci
JT  - Analytical sciences : the international journal of the Japan Society for 
      Analytical Chemistry
JID - 8511078
RN  - 0 (Acrylic Resins)
RN  - 0 (Proteins)
RN  - 0 (Solutions)
RN  - 368GB5141J (Sodium Dodecyl Sulfate)
RN  - 9003-05-8 (polyacrylamide)
RN  - 9004-67-5 (Methylcellulose)
RN  - 9011-14-7 (Polymethyl Methacrylate)
SB  - IM
MH  - Acrylic Resins/*chemistry
MH  - Adsorption
MH  - Electrophoresis, Microchip/*methods
MH  - Methylcellulose/chemistry
MH  - Microarray Analysis/instrumentation/*methods
MH  - Molecular Weight
MH  - Particle Size
MH  - Polymethyl Methacrylate/*chemistry
MH  - Proteins/*analysis
MH  - Sensitivity and Specificity
MH  - Sodium Dodecyl Sulfate/analysis
MH  - Solutions/chemistry
MH  - Surface Properties
MH  - Time Factors
EDAT- 2008/03/12 09:00
MHDA- 2008/08/05 09:00
CRDT- 2008/03/12 09:00
PHST- 2008/03/12 09:00 [pubmed]
PHST- 2008/08/05 09:00 [medline]
PHST- 2008/03/12 09:00 [entrez]
AID - JST.JSTAGE/analsci/24.321 [pii]
AID - 10.2116/analsci.24.321 [doi]
PST - ppublish
SO  - Anal Sci. 2008 Mar;24(3):321-5. doi: 10.2116/analsci.24.321.