PMID- 18335160
OWN - NLM
STAT- MEDLINE
DCOM- 20081105
LR  - 20211020
IS  - 0300-9564 (Print)
IS  - 0300-9564 (Linking)
VI  - 115
IP  - 7
DP  - 2008 Jul
TI  - Neurotrophin and neuropeptide expression in mouse brain is regulated by knockout 
      of the norepinephrine transporter.
PG  - 973-82
LID - 10.1007/s00702-008-0039-2 [doi]
AB  - Neurotrophins [e.g. nerve growth factor (NGF), brain-derived neurotrophic factor 
      (BDNF) and neurotrophin-3 (NT-3)] and neuropeptides such as 
      corticotropin-releasing factor (CRF) are reported to contribute to the action of 
      antidepressants (ADs). Norepinephrine transporter (NET) knockout (NETKO) mice 
      represent a model of chronic AD treatment. In the present study, we examined 
      brain-region-specific regulations of NT-3, NGF, BDNF and CRF at the mRNA and 
      protein level in NET wild-type (NETWT) and NETKO mice by means of quantitative 
      real-time PCR (qPCR) and two-site enzyme-linked immunosorbent assays (ELISAs), 
      respectively. NETKO-induced changes were detected for NT-3 in olfactory bulb, 
      brainstem and whole brain at the mRNA and for olfactory bulb at the protein 
      level, for NGF mRNA and protein in olfactory bulb, cerebellum and brainstem and 
      for CRF mRNA and protein in the hippocampus. In contrast, BDNF levels remained 
      unaltered. Our results suggest that NETKO mice represent a useful model to 
      examine gene regulation of downstream targets potentially involved in the action 
      of ADs. We could delineate NT-3, NGF and CRF as being regulated in distinct brain 
      regions by KO of the NET.
FAU - Haenisch, B
AU  - Haenisch B
AD  - Institute of Pharmacology and Toxicology, University of Bonn, Reuterstrasse 2b, 
      53113 Bonn, Germany.
FAU - Gilsbach, R
AU  - Gilsbach R
FAU - Bonisch, H
AU  - Bonisch H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080312
PL  - Austria
TA  - J Neural Transm (Vienna)
JT  - Journal of neural transmission (Vienna, Austria : 1996)
JID - 9702341
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neuropeptides)
RN  - 0 (Norepinephrine Plasma Membrane Transport Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Brain/anatomy & histology/*metabolism
MH  - Gene Expression Regulation/*genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Nerve Growth Factors/genetics/*metabolism
MH  - Neuropeptides/genetics/*metabolism
MH  - Norepinephrine Plasma Membrane Transport Proteins/*deficiency
MH  - RNA, Messenger/metabolism
EDAT- 2008/03/13 09:00
MHDA- 2008/11/06 09:00
CRDT- 2008/03/13 09:00
PHST- 2007/10/23 00:00 [received]
PHST- 2008/02/26 00:00 [accepted]
PHST- 2008/03/13 09:00 [pubmed]
PHST- 2008/11/06 09:00 [medline]
PHST- 2008/03/13 09:00 [entrez]
AID - 10.1007/s00702-008-0039-2 [doi]
PST - ppublish
SO  - J Neural Transm (Vienna). 2008 Jul;115(7):973-82. doi: 10.1007/s00702-008-0039-2. 
      Epub 2008 Mar 12.