PMID- 18336924
OWN - NLM
STAT- MEDLINE
DCOM- 20080612
LR  - 20080408
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 149
IP  - 2
DP  - 2008 May
TI  - Generation of internal image monoclonal anti-idiotypic antibodies against 
      idiotypic antibodies to GP5 antigen of porcine reproductive and respiratory 
      syndrome virus.
PG  - 300-8
LID - 10.1016/j.jviromet.2008.01.010 [doi]
AB  - Syngeneic monoclonal anti-idiotypic antibodies (Mab2s) were generated against 
      idiotypic antibodies to membrane glycoprotein GP5 of porcine reproductive and 
      respiratory syndrome virus (PRRSV) using the sequential immunization method. Six 
      of 12 Mab2s possessed potential internal image characteristics by recognizing a 
      common idiotype on murine and swine anti-GP5 antibodies. Further serological 
      characterization demonstrated that one of the Mab2 (Mab2-5G2) represents internal 
      image anti-idiotope which mimicked the GP5 antigen that inhibited the interaction 
      between idiotypic anti-GP5 antibodies and GP5 antigen, its reaction with the 
      idiotypic anti-GP5 antibody was inhibited by GP5 antigen and detected the common 
      Id on anti-PRRSV antibodies from pigs that were experimentally infected with 
      PRRSV. In addition, Mab2-5G2 identified a soluble protein on MA-104 and porcine 
      alveolar macrophages. These results indicate that Mab2-5G2 may be a useful 
      candidate as an alternative PRRSV serodiagnostic reagent and a useful probe to 
      study PRRSV-cell interaction.
FAU - Zhou, En-Min
AU  - Zhou EM
AD  - Department of Preventive Veterinary Medicine, College of Animal Science and 
      Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, TaiAn, 
      Shandong 271018, China. zhouem@sdau.edu.cn
FAU - Xiao, Yihong
AU  - Xiao Y
FAU - Shi, Youfei
AU  - Shi Y
FAU - Li, Xiaojing
AU  - Li X
FAU - Ma, Lihong
AU  - Ma L
FAU - Jing, Shengtao
AU  - Jing S
FAU - Peng, Jun
AU  - Peng J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080311
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Antibodies, Anti-Idiotypic/*immunology/*isolation & purification
MH  - Antibodies, Monoclonal/immunology/isolation & purification
MH  - Antibodies, Viral/blood/*immunology
MH  - Antigens, Viral/immunology
MH  - Cell Line
MH  - Cells, Cultured
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Female
MH  - Macrophages, Alveolar/immunology
MH  - Mice
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
MH  - Viral Envelope Proteins/*immunology
EDAT- 2008/03/14 09:00
MHDA- 2008/06/13 09:00
CRDT- 2008/03/14 09:00
PHST- 2007/11/04 00:00 [received]
PHST- 2007/12/23 00:00 [revised]
PHST- 2008/01/10 00:00 [accepted]
PHST- 2008/03/14 09:00 [pubmed]
PHST- 2008/06/13 09:00 [medline]
PHST- 2008/03/14 09:00 [entrez]
AID - S0166-0934(08)00024-4 [pii]
AID - 10.1016/j.jviromet.2008.01.010 [doi]
PST - ppublish
SO  - J Virol Methods. 2008 May;149(2):300-8. doi: 10.1016/j.jviromet.2008.01.010. Epub 
      2008 Mar 11.