PMID- 18337560
OWN - NLM
STAT- MEDLINE
DCOM- 20080710
LR  - 20211020
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 111
IP  - 10
DP  - 2008 May 15
TI  - IgG regulates the CD1 expression profile and lipid antigen-presenting function in 
      human dendritic cells via FcgammaRIIa.
PG  - 5037-46
LID - 10.1182/blood-2007-07-099549 [doi]
AB  - Dendritic cells (DCs) process and present bacterial and endogenous lipid antigens 
      in complex with CD1 molecules to T cells and invariant natural killer T (NKT) 
      cells. However, different types of DCs, such as blood myeloid DCs and skin 
      Langerhans cells, exhibit distinct patterns of CD1a, CD1b, CD1c, and CD1d 
      expression. The regulation of such differences is incompletely understood. Here, 
      we initially observed that monocyte-derived DCs cultured in an 
      immunoglobulin-rich milieu expressed CD1d but not CD1a, CD1b, and CD1c, whereas 
      DCs cultured in the presence of low levels of immunoglobulins had an opposite CD1 
      profile. Based on this, we tested the possibility that immunoglobulins play a 
      central role in determining these differences. IgG depletion and intravenous 
      immunoglobulin (IVIg) add-in experiments strongly supported a role for IgG in 
      directing the CD1 expression profile. Blocking experiments indicated that this 
      effect was mediated by FcgammaRIIa (CD32a), and quantitative polymerase chain 
      reaction data demonstrated that regulation of the CD1 profile occurred at the 
      gene expression level. Finally, the ability of DCs to activate CD1-restricted NKT 
      cells and T cells was determined by this regulatory effect of IgG. Our data 
      demonstrate an important role for FcgammaRIIa in regulating the CD1 antigen 
      presentation machinery of human DCs.
FAU - Smed-Sorensen, Anna
AU  - Smed-Sorensen A
AD  - Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, 
      Stockholm, Sweden.
FAU - Moll, Markus
AU  - Moll M
FAU - Cheng, Tan-Yun
AU  - Cheng TY
FAU - Lore, Karin
AU  - Lore K
FAU - Norlin, Anna-Carin
AU  - Norlin AC
FAU - Perbeck, Leif
AU  - Perbeck L
FAU - Moody, D Branch
AU  - Moody DB
FAU - Spetz, Anna-Lena
AU  - Spetz AL
FAU - Sandberg, Johan K
AU  - Sandberg JK
LA  - eng
GR  - AI52731/AI/NIAID NIH HHS/United States
GR  - R01 AI052731/AI/NIAID NIH HHS/United States
GR  - R37 AI052731/AI/NIAID NIH HHS/United States
GR  - AR 048632/AR/NIAMS NIH HHS/United States
GR  - R01 AI049313/AI/NIAID NIH HHS/United States
GR  - R01 AR048632/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080312
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD1)
RN  - 0 (Fc gamma receptor IIA)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Receptors, IgG)
SB  - IM
MH  - Antigens, CD/*physiology
MH  - Antigens, CD1/*genetics
MH  - Cell Culture Techniques
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/*immunology
MH  - Gene Expression Regulation/immunology
MH  - Humans
MH  - Immunoglobulin G/*pharmacology
MH  - Killer Cells, Natural
MH  - Lymphocyte Activation
MH  - Receptors, IgG/*physiology
MH  - T-Lymphocytes
PMC - PMC2384132
EDAT- 2008/03/14 09:00
MHDA- 2008/07/11 09:00
PMCR- 2009/05/15
CRDT- 2008/03/14 09:00
PHST- 2008/03/14 09:00 [pubmed]
PHST- 2008/07/11 09:00 [medline]
PHST- 2008/03/14 09:00 [entrez]
PHST- 2009/05/15 00:00 [pmc-release]
AID - S0006-4971(20)47188-5 [pii]
AID - 2007/099549 [pii]
AID - 10.1182/blood-2007-07-099549 [doi]
PST - ppublish
SO  - Blood. 2008 May 15;111(10):5037-46. doi: 10.1182/blood-2007-07-099549. Epub 2008 
      Mar 12.