PMID- 18337615
OWN - NLM
STAT- MEDLINE
DCOM- 20080805
LR  - 20220311
IS  - 0022-2275 (Print)
IS  - 0022-2275 (Linking)
VI  - 49
IP  - 6
DP  - 2008 Jun
TI  - Phosphatidylserine prevents UV-induced decrease of type I procollagen and 
      increase of MMP-1 in dermal fibroblasts and human skin in vivo.
PG  - 1235-45
LID - 10.1194/jlr.M700581-JLR200 [doi]
AB  - In an effort to find topical agents that prevent or retard cutaneous aging, seven 
      functional lipids were screened for their procollagen-upregulating and matrix 
      metalloproteinase (MMP)-1-downregulating activities in human dermal fibroblasts 
      by Western blotting. The preventive effect on ultraviolet (UV)-induced decrease 
      of procollagen was demonstrated in phosphatidylserine (PS), 
      lysophosphatidylserine (LPS), lysophosphatidic acid (LPA), N-acetyl 
      phytosphingosine (NAPS), and tetraacetyl phytosphingosine (TAPS). Furthermore, 
      PS, LPS, and LPA upregulated procollagen expression in unirradiated basal 
      conditions. The inhibitory effect on UV-induced MMP-1 expression was seen in 
      NAPS, TAPS, LPA, PS, lysophosphatidylglycerol, and LPS. PS was chosen as the most 
      suitable candidate anti-aging chemical for the subsequent in vivo studies. We 
      investigated the effects of PS on acute UV response and chronologic skin aging by 
      topically applying it to young skin before UV irradiation and to aged human skin, 
      respectively. Real-time PCR and Western blot revealed that in the young skin, PS 
      treatment prevented UV-induced reduction in procollagen expression and inhibited 
      UV-induced MMP-1 expression. PS also blocked UV-induced IL-6 and COX-2 gene 
      expression in cultured fibroblasts dose-dependently. In the aged skin, PS caused 
      increased procollagen transcription and procollagen immunostaining in the upper 
      dermis, and a significant decrease in MMP-1 expression at both mRNA and protein 
      levels. These results indicate that topical PS has anti-skin-aging properties and 
      point to the potential use of PS as a therapeutic agent in the prevention and 
      treatment of cutaneous aging.
FAU - Cho, Soyun
AU  - Cho S
AD  - Department of Dermatology, Seoul National University College of Medicine, Seoul, 
      Korea.
FAU - Kim, Hyeon Ho
AU  - Kim HH
FAU - Lee, Min Jung
AU  - Lee MJ
FAU - Lee, Serah
AU  - Lee S
FAU - Park, Chang-Seo
AU  - Park CS
FAU - Nam, Sang-June
AU  - Nam SJ
FAU - Han, Jeong-Jun
AU  - Han JJ
FAU - Kim, Jin-Wook
AU  - Kim JW
FAU - Chung, Jin Ho
AU  - Chung JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080312
PL  - United States
TA  - J Lipid Res
JT  - Journal of lipid research
JID - 0376606
RN  - 0 (Collagen Type I)
RN  - 0 (DNA Primers)
RN  - 0 (Interleukin-1alpha)
RN  - 0 (Interleukin-6)
RN  - 0 (Phosphatidylserines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Base Sequence
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Collagen Type I/genetics/*metabolism
MH  - Cyclooxygenase 2/genetics
MH  - DNA Primers
MH  - Fibroblasts/enzymology/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Interleukin-1alpha/metabolism
MH  - Interleukin-6/genetics
MH  - Matrix Metalloproteinase 1/genetics/*metabolism
MH  - Phosphatidylserines/*pharmacology
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/genetics
MH  - Skin/cytology/enzymology/*metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - *Ultraviolet Rays
EDAT- 2008/03/14 09:00
MHDA- 2008/08/06 09:00
CRDT- 2008/03/14 09:00
PHST- 2008/03/14 09:00 [pubmed]
PHST- 2008/08/06 09:00 [medline]
PHST- 2008/03/14 09:00 [entrez]
AID - S0022-2275(20)42368-5 [pii]
AID - 10.1194/jlr.M700581-JLR200 [doi]
PST - ppublish
SO  - J Lipid Res. 2008 Jun;49(6):1235-45. doi: 10.1194/jlr.M700581-JLR200. Epub 2008 
      Mar 12.