PMID- 18338249
OWN - NLM
STAT- MEDLINE
DCOM- 20081031
LR  - 20211020
IS  - 1573-6903 (Electronic)
IS  - 0364-3190 (Print)
IS  - 0364-3190 (Linking)
VI  - 33
IP  - 9
DP  - 2008 Sep
TI  - Transcription factor GATA-3 regulates the transcriptional activity of dopamine 
      beta-hydroxylase by interacting with Sp1 and AP4.
PG  - 1821-31
LID - 10.1007/s11064-008-9639-3 [doi]
AB  - GATA-3 is a zinc finger transcription factor that is expressed in T cell lineages 
      as well as in the nervous system during development. In this study, we report 
      that forced expression of GATA-3 resulted in an increased number of dopamine 
      beta-hydroxylase (DBH)-expressing neurons in primary neural crest stem cell 
      (NCSC) culture, suggesting that the DBH gene may be a downstream target gene of 
      GATA-3. GATA-3 robustly transactivates the promoter function of the noradrenaline 
      (NA)-synthesizing DBH gene, via two specific upstream promoter domains; one at 
      -62 to -32 bp and the other at -891 to -853 bp. Surprisingly, none of these 
      domains contain GATA-3 binding sites but encompass binding motifs for 
      transcription factors Sp1 and AP4, respectively. Protein-protein interaction 
      analyses both in vitro and in vivo and chromatin immunoprecipitation (ChIP) 
      assays showed that GATA-3 effects its transcriptional regulatory function through 
      physical interactions with these transcription factors.
FAU - Hong, Seok Jong
AU  - Hong SJ
AD  - Molecular Neurobiology Laboratory, MRC215, McLean Hospital, Harvard Medical 
      School, 115 Mill Street, Belmont, MA, 02478, USA.
FAU - Choi, Hyun Jin
AU  - Choi HJ
FAU - Hong, Sunghoi
AU  - Hong S
FAU - Huh, Youngbuhm
AU  - Huh Y
FAU - Chae, Han
AU  - Chae H
FAU - Kim, Kwang-Soo
AU  - Kim KS
LA  - eng
GR  - R01 MH048866/MH/NIMH NIH HHS/United States
GR  - R01 MH048866-17/MH/NIMH NIH HHS/United States
GR  - R01 NS084869/NS/NINDS NIH HHS/United States
GR  - R29 MH048866/MH/NIMH NIH HHS/United States
GR  - R01 DC006501/DC/NIDCD NIH HHS/United States
GR  - MH48866/MH/NIMH NIH HHS/United States
GR  - DC006501/DC/NIDCD NIH HHS/United States
GR  - R01 DC006501-05/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20080313
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (GATA3 Transcription Factor)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Sp1 Transcription Factor)
RN  - 0 (Transcription Factors)
RN  - EC 1.14.17.1 (Dopamine beta-Hydroxylase)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Dopamine beta-Hydroxylase/genetics/*metabolism
MH  - GATA3 Transcription Factor/genetics/*metabolism
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Neurons/cytology/physiology
MH  - Promoter Regions, Genetic
MH  - Quail
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Sp1 Transcription Factor/genetics/*metabolism
MH  - Transcription Factors/genetics/*metabolism
MH  - *Transcription, Genetic
PMC - PMC2712938
MID - NIHMS123429
EDAT- 2008/03/14 09:00
MHDA- 2008/11/01 09:00
PMCR- 2009/07/20
CRDT- 2008/03/14 09:00
PHST- 2007/12/20 00:00 [received]
PHST- 2008/02/20 00:00 [accepted]
PHST- 2008/03/14 09:00 [pubmed]
PHST- 2008/11/01 09:00 [medline]
PHST- 2008/03/14 09:00 [entrez]
PHST- 2009/07/20 00:00 [pmc-release]
AID - 10.1007/s11064-008-9639-3 [doi]
PST - ppublish
SO  - Neurochem Res. 2008 Sep;33(9):1821-31. doi: 10.1007/s11064-008-9639-3. Epub 2008 
      Mar 13.