PMID- 18338783
OWN - NLM
STAT- MEDLINE
DCOM- 20080729
LR  - 20220317
IS  - 1099-1573 (Electronic)
IS  - 0951-418X (Linking)
VI  - 22
IP  - 4
DP  - 2008 Apr
TI  - Hypoglycemic effect of a novel dialysed fenugreek seeds extract is sustainable 
      and is mediated, in part, by the activation of hepatic enzymes.
PG  - 500-5
LID - 10.1002/ptr.2351 [doi]
AB  - A novel preparation of a dialysed aqueous extract of fenugreek seeds (FSE) that 
      stimulates the insulin signalling pathway was reported previously (Vijayakumar et 
      al., 2005). The present study was designed to investigate the long-term effects 
      (multiple dose effect) of this FSE preparation on the blood glucose level and 
      body weight, and a short-term effect (single dose effect) on serum insulin and 
      hepatic enzymes, in experimentally induced diabetic conditions. The multiple dose 
      effect of FSE on the glucose level and body weight was studied in alloxan 
      (AXN)-diabetic mice in comparison with the vehicle treated control diabetic mice. 
      Intraperitoneal (i.p.) administration of FSE (15 mg/kg body weight (BW)) for 5 
      consecutive days reduced hyperglycemia in AXN-diabetic mice on day 5 and this 
      effect was further sustained for 10 days. The FSE-induced hypoglycemic effect was 
      accompanied without any reduction in the body weight compared with the diabetic 
      mice in which the body weight was reduced significantly. A single dose effect of 
      FSE on hepatic glucokinase (GK) and hexokinase (HK) enzymes was studied in 
      streptozotocin (STZ)-diabetic mice. Intraperitoneal administration of FSE (15 
      mg/kg BW) by 90 min decreased the blood glucose levels significantly (p < 0.01) 
      in STZ-diabetic mice and the effect was comparable to that achieved by insulin 
      (1.5 U/kg BW) injection. This effect was associated with a significant 
      enhancement in the liver GK and HK activities on a par with that of insulin. In 
      normal glucose loaded mice, FSE improved the intraperitoneal glucose tolerance 
      accompanied by a reduction in serum insulin concentration. These results are 
      indicative of an extra-pancreatic mode of action of FSE. The present study 
      concludes that this novel FSE preparation corrects metabolic alterations 
      associated with diabetes by exhibiting insulin-like properties and has a 
      potential for clinical applications.
CI  - (c) 2008 John Wiley & Sons, Ltd.
FAU - Vijayakumar, Maleppillil Vavachan
AU  - Vijayakumar MV
AD  - National Centre for Cell Science, NCCS Complex, Ganeshkhind, Pune 411 007, India.
FAU - Bhat, Manoj Kumar
AU  - Bhat MK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Phytother Res
JT  - Phytotherapy research : PTR
JID - 8904486
RN  - 0 (Blood Glucose)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Plant Extracts)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.2 (Glucokinase)
SB  - IM
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - Diabetes Mellitus, Experimental/blood/metabolism/prevention & control
MH  - Enzyme Activation/drug effects
MH  - Glucokinase/metabolism
MH  - Glucose Tolerance Test
MH  - Hexokinase/metabolism
MH  - Hypoglycemic Agents/administration & dosage/*pharmacology
MH  - Injections, Intraperitoneal
MH  - Liver/*drug effects/enzymology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Plant Extracts/administration & dosage/*pharmacology
MH  - Seeds/*chemistry
MH  - Trigonella/*chemistry
EDAT- 2008/03/15 09:00
MHDA- 2008/07/30 09:00
CRDT- 2008/03/15 09:00
PHST- 2008/03/15 09:00 [pubmed]
PHST- 2008/07/30 09:00 [medline]
PHST- 2008/03/15 09:00 [entrez]
AID - 10.1002/ptr.2351 [doi]
PST - ppublish
SO  - Phytother Res. 2008 Apr;22(4):500-5. doi: 10.1002/ptr.2351.