PMID- 18338926
OWN - NLM
STAT- MEDLINE
DCOM- 20080701
LR  - 20220409
IS  - 1130-1406 (Print)
IS  - 1130-1406 (Linking)
VI  - 25
IP  - 1
DP  - 2008 Mar
TI  - A seed and feed model for the formation of Candida albicans biofilms under flow 
      conditions using an improved modified Robbins device.
PG  - 37-40
AB  - A variety of manifestations of Candida albicans infections are associated with 
      the formation of biofilms on the surface of biomaterials. In order to maintain 
      their niche these adherent populations need to withstand the continuous bathing 
      action of physiological fluids (saliva, blood), which also provide water and 
      nutrients to the fungal cells. Thus, it was the aim of this study to examine and 
      further characterize the development of C. albicans biofilms under shear forces 
      and a flow of replenishing nutrients, emulating the conditions that fungal cells 
      would normally encounter within the host. An improved modified Robbins device 
      (MRD) was designed to hold six poly methyl methacrylate (PMMA) plugs of 25 mm in 
      diameter. A "seed and feed" model of biofilm formation was then implemented for 
      which the apparatus was initially seeded with a C. albicans cell suspension to 
      allow initial adhesion of fungal cells to the biomaterial. Following this initial 
      step, sterile medium was then pumped through the MRD at a constant flow rate. 
      Scanning electron microscopy (SEM) and confocal scanning laser microscopy (CSLM) 
      demonstrated a high degree of heterogeneity associated with the structure of 
      biofilms formed under flowing conditions using the MRD. In addition, these 
      biofilms displayed a complex three dimensional architecture and increased 
      production of exopolymeric material.
FAU - Ramage, Gordon
AU  - Ramage G
AD  - Department of Biology and South Texas Center for Emerging Infectious Diseases, 
      The University of Texas at San Antonio, San Antonio, Texas 78249, USA.
FAU - Wickes, Brian L
AU  - Wickes BL
FAU - Lopez-Ribot, Jose L
AU  - Lopez-Ribot JL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Spain
TA  - Rev Iberoam Micol
JT  - Revista iberoamericana de micologia
JID - 9425531
RN  - 0 (Culture Media)
RN  - 9011-14-7 (Polymethyl Methacrylate)
SB  - IM
MH  - Biofilms/*growth & development
MH  - Candida albicans/*drug effects/ultrastructure
MH  - Culture Media/pharmacology
MH  - Equipment Design
MH  - Microscopy, Confocal
MH  - Microscopy, Electron, Scanning
MH  - *Models, Biological
MH  - Mycology/*instrumentation
MH  - Polymethyl Methacrylate
MH  - *Rheology
EDAT- 2008/03/15 09:00
MHDA- 2008/07/02 09:00
CRDT- 2008/03/15 09:00
PHST- 2008/03/15 09:00 [pubmed]
PHST- 2008/07/02 09:00 [medline]
PHST- 2008/03/15 09:00 [entrez]
AID - 20082537 [pii]
AID - 10.1016/s1130-1406(08)70009-3 [doi]
PST - ppublish
SO  - Rev Iberoam Micol. 2008 Mar;25(1):37-40. doi: 10.1016/s1130-1406(08)70009-3.