PMID- 18338954
OWN - NLM
STAT- MEDLINE
DCOM- 20080904
LR  - 20211020
IS  - 1536-2302 (Print)
IS  - 1557-7457 (Electronic)
IS  - 1536-2302 (Linking)
VI  - 10
IP  - 2
DP  - 2008 Jun
TI  - Embryonic stem cells contribute to mouse chimeras in the absence of detectable 
      cell fusion.
PG  - 231-48
LID - 10.1089/clo.2007.0039 [doi]
AB  - Embryonic stem (ES) cells are capable of differentiating into all embryonic and 
      adult cell types following mouse chimera production. Although injection of 
      diploid ES cells into tetraploid blastocysts suggests that tetraploid cells have 
      a selective disadvantage in the developing embryo, tetraploid hybrid cells, 
      formed by cell fusion between ES cells and somatic cells, have been reported to 
      contribute to mouse chimeras. In addition, other examples of apparent stem cell 
      plasticity have recently been shown to be the result of cell fusion. Here we 
      investigate whether ES cells contribute to mouse chimeras through a cell fusion 
      mechanism. Fluorescence in situ hybridization (FISH) analysis for X and Y 
      chromosomes was performed on dissociated tissues from embryonic, neonatal, and 
      adult wild-type, and chimeric mice to follow the ploidy distributions of cells 
      from various tissues. FISH analysis showed that the ploidy distributions in 
      dissociated tissues, notably the tetraploid cell number, did not differ between 
      chimeric and wild-type tissues. To address the possibility that early cell fusion 
      events are hidden by subsequent reductive divisions or other changes in cell 
      ploidy, we injected Z/EG (lacZ/EGFP) ES cells into ACTB-cre blastocysts. 
      Recombination can only occur as the result of cell fusion, and the recombined 
      allele should persist through any subsequent changes in cell ploidy. We did not 
      detect evidence of fusion in embryonic chimeras either by direct fluorescence 
      microscopy for GFP or by PCR amplification of the recombined Z/EG locus on 
      genomic DNA from ACTB-cre::Z/EG chimeric embryos. Our results argue strongly 
      against cell fusion as a mechanism by which ES cells contribute to chimeras.
FAU - Kidder, Benjamin L
AU  - Kidder BL
AD  - Department of Medicine, Stem Cell Institute, Minneapolis, Minnesota, USA.
FAU - Oseth, Leann
AU  - Oseth L
FAU - Miller, Shanna
AU  - Miller S
FAU - Hirsch, Betsy
AU  - Hirsch B
FAU - Verfaillie, Catherine
AU  - Verfaillie C
FAU - Coucouvanis, Electra
AU  - Coucouvanis E
LA  - eng
GR  - 5R01-DK058295-05/DK/NIDDK NIH HHS/United States
GR  - 5R01-HL067932-03/HL/NHLBI NIH HHS/United States
GR  - P30 CA077598-09/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cloning Stem Cells
JT  - Cloning and stem cells
JID - 101125444
RN  - 0 (Recombinases)
SB  - IM
MH  - Animals
MH  - Blastocyst/physiology
MH  - *Cell Fusion
MH  - *Chimera
MH  - Embryo, Mammalian/embryology/metabolism
MH  - Embryonic Stem Cells/*physiology
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Muscle, Skeletal/cytology
MH  - Neuroepithelial Cells/cytology
MH  - Ploidies
MH  - Recombinases/metabolism
MH  - Recombination, Genetic
MH  - Transfection
MH  - Transgenes
MH  - X Chromosome
MH  - Y Chromosome
PMC - PMC2981375
EDAT- 2008/03/15 09:00
MHDA- 2008/09/05 09:00
PMCR- 2009/06/01
CRDT- 2008/03/15 09:00
PHST- 2008/03/15 09:00 [pubmed]
PHST- 2008/09/05 09:00 [medline]
PHST- 2008/03/15 09:00 [entrez]
PHST- 2009/06/01 00:00 [pmc-release]
AID - 10.1089/clo.2007.0039 [pii]
AID - 10.1089/clo.2007.0039 [doi]
PST - ppublish
SO  - Cloning Stem Cells. 2008 Jun;10(2):231-48. doi: 10.1089/clo.2007.0039.