PMID- 18339401
OWN - NLM
STAT- MEDLINE
DCOM- 20080701
LR  - 20231213
IS  - 1089-8638 (Electronic)
IS  - 0022-2836 (Linking)
VI  - 377
IP  - 5
DP  - 2008 Apr 11
TI  - Subtle changes in peptide conformation profoundly affect recognition of the 
      non-classical MHC class I molecule HLA-E by the CD94-NKG2 natural killer cell 
      receptors.
PG  - 1297-303
LID - 10.1016/j.jmb.2008.01.098 [doi]
AB  - Human leukocyte antigen (HLA)-E is a non-classical major histocompatibility 
      complex class I molecule that binds peptides derived from the leader sequences of 
      other HLA class I molecules. Natural killer cell recognition of these HLA-E 
      molecules, via the CD94-NKG2 natural killer family, represents a central innate 
      mechanism for monitoring major histocompatibility complex expression levels 
      within a cell. The leader sequence-derived peptides bound to HLA-E exhibit very 
      limited polymorphism, yet subtle differences affect the recognition of HLA-E by 
      the CD94-NKG2 receptors. To better understand the basis for this peptide-specific 
      recognition, we determined the structure of HLA-E in complex with two leader 
      peptides, namely, HLA-Cw*07 (VMAPRALLL), which is poorly recognised by CD94-NKG2 
      receptors, and HLA-G*01 (VMAPRTLFL), a high-affinity ligand of CD94-NKG2 
      receptors. A comparison of these structures, both of which were determined to 
      2.5-A resolution, revealed that allotypic variations in the bound leader 
      sequences do not result in conformational changes in the HLA-E heavy chain, 
      although subtle changes in the conformation of the peptide within the binding 
      groove of HLA-E were evident. Accordingly, our data indicate that the CD94-NKG2 
      receptors interact with HLA-E in a manner that maximises the ability of the 
      receptors to discriminate between subtle changes in both the sequence and 
      conformation of peptides bound to HLA-E.
FAU - Hoare, Hilary L
AU  - Hoare HL
AD  - The Protein Crystallography Unit, Department of Biochemistry and Molecular 
      Biology, School of Biomedical Sciences, Monash University, Clayton, Victoria 
      3800, Australia.
FAU - Sullivan, Lucy C
AU  - Sullivan LC
FAU - Clements, Craig S
AU  - Clements CS
FAU - Ely, Lauren K
AU  - Ely LK
FAU - Beddoe, Travis
AU  - Beddoe T
FAU - Henderson, Kate N
AU  - Henderson KN
FAU - Lin, Jie
AU  - Lin J
FAU - Reid, Hugh H
AU  - Reid HH
FAU - Brooks, Andrew G
AU  - Brooks AG
FAU - Rossjohn, Jamie
AU  - Rossjohn J
LA  - eng
SI  - PDB/3BZE
SI  - PDB/3BZF
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20080212
PL  - Netherlands
TA  - J Mol Biol
JT  - Journal of molecular biology
JID - 2985088R
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (NK Cell Lectin-Like Receptor Subfamily D)
RN  - 0 (Receptors, Immunologic)
SB  - IM
MH  - HLA Antigens/chemistry/*immunology
MH  - Histocompatibility Antigens Class I/chemistry/*immunology
MH  - Humans
MH  - Immunity, Innate/immunology
MH  - Killer Cells, Natural/immunology/metabolism
MH  - NK Cell Lectin-Like Receptor Subfamily D/*immunology
MH  - Protein Conformation
MH  - Receptors, Immunologic/*immunology/metabolism
MH  - HLA-E Antigens
RF  - 30
EDAT- 2008/03/15 09:00
MHDA- 2008/07/02 09:00
CRDT- 2008/03/15 09:00
PHST- 2007/11/05 00:00 [received]
PHST- 2008/01/18 00:00 [revised]
PHST- 2008/01/22 00:00 [accepted]
PHST- 2008/03/15 09:00 [pubmed]
PHST- 2008/07/02 09:00 [medline]
PHST- 2008/03/15 09:00 [entrez]
AID - S0022-2836(08)00177-0 [pii]
AID - 10.1016/j.jmb.2008.01.098 [doi]
PST - ppublish
SO  - J Mol Biol. 2008 Apr 11;377(5):1297-303. doi: 10.1016/j.jmb.2008.01.098. Epub 
      2008 Feb 12.