PMID- 18339684
OWN - NLM
STAT- MEDLINE
DCOM- 20080801
LR  - 20220408
IS  - 1460-2180 (Electronic)
IS  - 0143-3334 (Linking)
VI  - 29
IP  - 5
DP  - 2008 May
TI  - Aberrations of chromosome 19 in asbestos-associated lung cancer and in 
      asbestos-induced micronuclei of bronchial epithelial cells in vitro.
PG  - 913-7
LID - 10.1093/carcin/bgn068 [doi]
AB  - Exposure to asbestos is known to induce lung cancer, and our previous studies 
      have suggested that specific chromosomal regions, such as 19p13, are 
      preferentially aberrant in lung tumours of asbestos-exposed patients. Here, we 
      further examined the association between the 19p region and exposure to asbestos 
      using array comparative genomic hybridization and fluorescence in situ 
      hybridization (FISH) in lung tumours and FISH characterization of 
      asbestos-induced micronuclei (MN) in human bronchial epithelial BEAS 2B cells in 
      vitro. We detected an increased number of 19p losses in the tumours of 
      asbestos-exposed patients in comparison with tumours from non-exposed subjects 
      with similar distribution of tumour histology in both groups (13/33; 39% versus 
      3/25; 12%, P = 0.04). In BEAS 2B cells, a 48 h exposure to crocidolite asbestos 
      (2.0 microg/cm(2)) was found to induce centromere-negative MN-harbouring 
      chromosomal fragments. Furthermore, an increased frequency of rare MN containing 
      a 19p fragment was observed after the crocidolite treatment in comparison with 
      untreated controls (6/6000 versus 1/10 000, P = 0.01). The results suggest that 
      19p has significance in asbestos-associated carcinogenesis and that asbestos may 
      be capable of inducing specific chromosome aberrations.
FAU - Ruosaari, Salla T
AU  - Ruosaari ST
AD  - Biological Mechanisms and Prevention of Work-related Diseases, Health and Work 
      Ability, Finnish Institute of Occupational Health, Topeliuksenkatu 41 aA, 
      FI-00250 Helsinki, Finland.
FAU - Nymark, Penny E H
AU  - Nymark PE
FAU - Aavikko, Mervi M
AU  - Aavikko MM
FAU - Kettunen, Eeva
AU  - Kettunen E
FAU - Knuutila, Sakari
AU  - Knuutila S
FAU - Hollmen, Jaakko
AU  - Hollmen J
FAU - Norppa, Hannu
AU  - Norppa H
FAU - Anttila, Sisko L
AU  - Anttila SL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080313
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
RN  - 1332-21-4 (Asbestos)
SB  - IM
MH  - Adenocarcinoma/chemically induced/genetics
MH  - Asbestos/analysis/*toxicity
MH  - Bronchi/drug effects/*pathology
MH  - Carcinoma, Small Cell/chemically induced/genetics
MH  - Carcinoma, Squamous Cell/chemically induced/genetics
MH  - Cells, Cultured
MH  - Chromosome Aberrations/*drug effects
MH  - Chromosomes, Artificial, Bacterial
MH  - Chromosomes, Human, Pair 19/*drug effects
MH  - Environmental Exposure
MH  - Epithelial Cells/drug effects/*pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/chemically induced/*genetics
MH  - Nucleic Acid Hybridization
MH  - Tumor Cells, Cultured
EDAT- 2008/03/15 09:00
MHDA- 2008/08/02 09:00
CRDT- 2008/03/15 09:00
PHST- 2008/03/15 09:00 [pubmed]
PHST- 2008/08/02 09:00 [medline]
PHST- 2008/03/15 09:00 [entrez]
AID - bgn068 [pii]
AID - 10.1093/carcin/bgn068 [doi]
PST - ppublish
SO  - Carcinogenesis. 2008 May;29(5):913-7. doi: 10.1093/carcin/bgn068. Epub 2008 Mar 
      13.