PMID- 18339698
OWN - NLM
STAT- MEDLINE
DCOM- 20080616
LR  - 20190722
IS  - 0009-9147 (Print)
IS  - 0009-9147 (Linking)
VI  - 54
IP  - 5
DP  - 2008 May
TI  - Tissue factor and monocyte chemoattractant protein-1 expression in hypertensive 
      individuals with normal or increased carotid intima-media wall thickness.
PG  - 814-23
LID - 10.1373/clinchem.2007.095547 [doi]
AB  - BACKGROUND: People with hypertension display an inflammatory pattern that 
      includes increased plasma concentrations of monocyte chemoattractant protein 1 
      (MCP-1) and C-reactive protein (CRP) and enhanced expression of tissue factor 
      (TF) mRNA in blood monocytes. METHODS: In this study, we investigated the 
      relationship between CRP concentrations and TF and MCP-1 mRNA expression in 
      unstimulated and lipopolysaccharide (LPS)-stimulated monocytes isolated from 
      hypertensives with or without an increase in carotid intima-media thickness 
      (IMT). We also investigated the expression of TF and MCP-1 mRNA and MCP-1 protein 
      after in vitro addition of CRP to monocytes. We measured CRP (by 
      immunonephelometry) and monocyte expression of TF and MCP-1 (by real-time PCR) in 
      80 untreated hypertensive patients without clinical cardiovascular disease (CVD) 
      or additional risk factors for CVD compared with 41 controls. Based on IMT 
      measured by carotid Doppler ultrasonography, patients were classified into the 
      categories of normal (< or =1 mm) or abnormal (>1 mm). TF and MCP-1 mRNA and 
      MCP-1 protein (by Western blotting) were measured after in vitro addition of CRP 
      to monocytes from 10 randomized controls as well as 10 hypertensives with IMT < 
      or =1 mm and 10 with IMT >1 mm. RESULTS: CRP and TF and MCP-1 mRNA concentrations 
      were significantly higher in IMT >1 mm hypertensives vs those with IMT < or =1 mm 
      and controls. CRP had no effect on monocyte TF mRNA from either hypertensives or 
      controls. CRP-stimulated monocytes from hypertensives, however, showed increased 
      MCP-1 mRNA and protein expression compared with controls and LPS-stimulated 
      cells. CONCLUSIONS: Our findings suggest that the inflammatory response of blood 
      monocytes plays an important role in the development of atherosclerosis and 
      hypertension.
FAU - Sardo, Maria A
AU  - Sardo MA
AD  - Department of Internal Medicine, University of Messina, Messina, Italy.
FAU - Campo, Salvatore
AU  - Campo S
FAU - Mandraffino, Giuseppe
AU  - Mandraffino G
FAU - Saitta, Carlo
AU  - Saitta C
FAU - Bonaiuto, Antonio
AU  - Bonaiuto A
FAU - Castaldo, Maria
AU  - Castaldo M
FAU - Cinquegrani, Maurizio
AU  - Cinquegrani M
FAU - Pizzimenti, Giovanni
AU  - Pizzimenti G
FAU - Saitta, Antonino
AU  - Saitta A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080313
PL  - England
TA  - Clin Chem
JT  - Clinical chemistry
JID - 9421549
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipopolysaccharides)
RN  - 9007-41-4 (C-Reactive Protein)
RN  - 9035-58-9 (Thromboplastin)
SB  - IM
MH  - Adult
MH  - C-Reactive Protein/metabolism
MH  - Carotid Arteries/diagnostic imaging/*pathology
MH  - Carotid Artery, Common/diagnostic imaging/pathology
MH  - Carotid Artery, Internal/diagnostic imaging/pathology
MH  - Carotid Stenosis/complications/diagnostic imaging/metabolism
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - Female
MH  - Humans
MH  - Hypertension/complications/*metabolism/pathology
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - Monocytes/drug effects/metabolism
MH  - Thromboplastin/*biosynthesis/genetics
MH  - Tunica Intima/diagnostic imaging/*pathology
MH  - Tunica Media/diagnostic imaging/*pathology
MH  - Ultrasonography
EDAT- 2008/03/15 09:00
MHDA- 2008/06/17 09:00
CRDT- 2008/03/15 09:00
PHST- 2008/03/15 09:00 [pubmed]
PHST- 2008/06/17 09:00 [medline]
PHST- 2008/03/15 09:00 [entrez]
AID - clinchem.2007.095547 [pii]
AID - 10.1373/clinchem.2007.095547 [doi]
PST - ppublish
SO  - Clin Chem. 2008 May;54(5):814-23. doi: 10.1373/clinchem.2007.095547. Epub 2008 
      Mar 13.