PMID- 18340473 OWN - NLM STAT- MEDLINE DCOM- 20080930 LR - 20211020 IS - 1367-5435 (Print) IS - 1367-5435 (Linking) VI - 35 IP - 7 DP - 2008 Jul TI - PVA-alginate immobilized cells for anaerobic ammonium oxidation (anammox) process. PG - 721-7 LID - 10.1007/s10295-008-0336-7 [doi] AB - The feasibility of an anaerobic ammonium oxidation (anammox) process combined with a cell-immobilization technique for autotrophic nitrogen removal was investigated. Anammox biomass was cultivated from local activated sludge and achieved significant anammox activity in 6 months. The development of a mature anammox biomass was confirmed by fluorescence in situ hybridization (FISH) analysis and off-line activity measurements. The abundance fraction of the anammox bacteria determined by FISH analysis was estimated by software. The anaerobic ammonia oxidizers occupied almost half of the total cells. Additionally, the anammox biomass was granulated as spherical gel beads of 3-4 mm in diameter by using a cell-immobilization technique. The nitrogen removal activity was proved to be successfully retained in the beads, with about 80% of nitrogenous compounds (NH(4) (+), NO(2) (- )and total nitrogen) removed after 48 h. These results offer a promising technique for the preservation of anammox microorganisms, the protection of them against the unfavorable surroundings, and the prevention of biomass washout towards the implementation of sustainable nitrogen elimination biotechnology. This is the first report on the immobilization of anammox biomass as gel beads. FAU - Hsia, Tsung-Hui AU - Hsia TH AD - Department of Safety Health and Environmental Engineering, Tungnan University, No.152, Sec.3, Pei-Shen Rd., Shen-Kun Village, Taipei, Taiwan, ROC. FAU - Feng, Yu-Jou AU - Feng YJ FAU - Ho, Chun-Ming AU - Ho CM FAU - Chou, Wen-Po AU - Chou WP FAU - Tseng, Szu-Kung AU - Tseng SK LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20080314 PL - Germany TA - J Ind Microbiol Biotechnol JT - Journal of industrial microbiology & biotechnology JID - 9705544 RN - 0 (Alginates) RN - 0 (Hexuronic Acids) RN - 0 (Nitrates) RN - 0 (Quaternary Ammonium Compounds) RN - 0 (Sewage) RN - 8A5D83Q4RW (Glucuronic Acid) RN - 9002-89-5 (Polyvinyl Alcohol) RN - N762921K75 (Nitrogen) SB - IM MH - *Alginates MH - Anaerobiosis MH - Bacteria/*metabolism MH - Cells, Immobilized/*metabolism MH - Glucuronic Acid MH - Hexuronic Acids MH - Microspheres MH - Nitrates/metabolism MH - Nitrogen/metabolism MH - Oxidation-Reduction MH - Polyvinyl Alcohol MH - Quaternary Ammonium Compounds/*metabolism MH - Sewage/microbiology EDAT- 2008/03/15 09:00 MHDA- 2008/10/01 09:00 CRDT- 2008/03/15 09:00 PHST- 2006/10/25 00:00 [received] PHST- 2008/02/21 00:00 [accepted] PHST- 2008/03/15 09:00 [pubmed] PHST- 2008/10/01 09:00 [medline] PHST- 2008/03/15 09:00 [entrez] AID - 10.1007/s10295-008-0336-7 [doi] PST - ppublish SO - J Ind Microbiol Biotechnol. 2008 Jul;35(7):721-7. doi: 10.1007/s10295-008-0336-7. Epub 2008 Mar 14.