PMID- 18341584
OWN - NLM
STAT- MEDLINE
DCOM- 20080613
LR  - 20240109
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 10
IP  - 6
DP  - 2008 Jun
TI  - Widespread distribution of a unique marine protistan lineage.
PG  - 1621-34
LID - 10.1111/j.1462-2920.2008.01580.x [doi]
AB  - Unicellular eukaryotes (protists) are key components of marine food webs, yet 
      knowledge of their diversity, distributions and respective ecologies is limited. 
      We investigated uncultured protists using 18S rRNA gene sequencing, phylogenetic 
      analyses, specific fluorescence in situ hybridization (FISH) probes and other 
      methods. Because few studies have been conducted in warm water systems, we 
      focused on two Atlantic subtropical regions, the Sargasso Sea and the Florida 
      Current. Cold temperate waters were also sampled. Gene sequences comprising a 
      unique eukaryotic lineage, herein termed 'biliphytes', were identified in most 
      samples, whether from high- (30 degrees C) or from low- (5 degrees C) temperature 
      waters. Sequences within this uncultured group have previously been retrieved 
      from high latitudes. Phylogenetic analyses suggest biliphytes are a sister group 
      to the cryptophytes and katablepharids, although the relationship is not 
      statistically supported. Bootstrap-supported subclades were delineated but 
      coherence was not obvious with respect to geography or physicochemical 
      parameters. Unlike results from the initial publication on these organisms 
      (therein 'picobiliphytes'), we could not detect a nucleomorph, either visually, 
      or by targeted primers. Phycobilin-like fluorescence associated with 
      biliphyte-specific FISH-probed cells supports the hypothesis that they are 
      photosynthetic. Our data indicate the biliphytes are nanoplanktonic in size, 
      averaging 4.1 +/- 1.0 x 3.5 +/- 0.8 microm (+/-SD) for one probed group, and 3.5 
      +/- 0.9 x 3.0 +/- 0.9 microm (+/-SD) for another. We estimate biliphytes 
      contributed 28 (+/-6)% of the phytoplanktonic biomass in tropical eddy-influenced 
      surface waters. Given their broad thermal and geographic distribution, 
      understanding the role these protists play in biogeochemical cycling within 
      different habitats is essential.
FAU - Cuvelier, Marie L
AU  - Cuvelier ML
AD  - Monterey Bay Aquarium Research Institute, 7700 Sandholdt Rd, Moss Landing, CA 
      95039, USA.
FAU - Ortiz, Alejandra
AU  - Ortiz A
FAU - Kim, Eunsoo
AU  - Kim E
FAU - Moehlig, Heike
AU  - Moehlig H
FAU - Richardson, David E
AU  - Richardson DE
FAU - Heidelberg, John F
AU  - Heidelberg JF
FAU - Archibald, John M
AU  - Archibald JM
FAU - Worden, Alexandra Z
AU  - Worden AZ
LA  - eng
SI  - GENBANK/EU368003
SI  - GENBANK/EU368004
SI  - GENBANK/EU368005
SI  - GENBANK/EU368006
SI  - GENBANK/EU368007
SI  - GENBANK/EU368008
SI  - GENBANK/EU368009
SI  - GENBANK/EU368010
SI  - GENBANK/EU368011
SI  - GENBANK/EU368012
SI  - GENBANK/EU368013
SI  - GENBANK/EU368014
SI  - GENBANK/EU368015
SI  - GENBANK/EU368016
SI  - GENBANK/EU368017
SI  - GENBANK/EU368018
SI  - GENBANK/EU368019
SI  - GENBANK/EU368020
SI  - GENBANK/EU368021
SI  - GENBANK/EU368022
SI  - GENBANK/EU368023
SI  - GENBANK/EU368024
SI  - GENBANK/EU368025
SI  - GENBANK/EU368026
SI  - GENBANK/EU368027
SI  - GENBANK/EU368028
SI  - GENBANK/EU368029
SI  - GENBANK/EU368030
SI  - GENBANK/EU368031
SI  - GENBANK/EU368032
SI  - GENBANK/EU368033
SI  - GENBANK/EU368034
SI  - GENBANK/EU368035
SI  - GENBANK/EU368036
SI  - GENBANK/EU368037
SI  - GENBANK/EU368038
SI  - GENBANK/EU368039
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20080312
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Protozoan)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Phycobilins)
RN  - 0 (RNA, Ribosomal, 18S)
SB  - IM
MH  - Animals
MH  - Atlantic Ocean
MH  - DNA Primers/genetics
MH  - DNA, Protozoan/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Eukaryota/*classification/cytology/genetics/*isolation & purification
MH  - Geography
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Photosynthesis
MH  - Phycobilins/genetics
MH  - Phylogeny
MH  - RNA, Ribosomal, 18S/genetics
MH  - Seawater/*parasitology
MH  - Sequence Analysis, DNA
PMC - PMC2408648
EDAT- 2008/03/18 09:00
MHDA- 2008/06/14 09:00
PMCR- 2008/05/31
CRDT- 2008/03/18 09:00
PHST- 2008/03/18 09:00 [pubmed]
PHST- 2008/06/14 09:00 [medline]
PHST- 2008/03/18 09:00 [entrez]
PHST- 2008/05/31 00:00 [pmc-release]
AID - EMI1580 [pii]
AID - 10.1111/j.1462-2920.2008.01580.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2008 Jun;10(6):1621-34. doi: 10.1111/j.1462-2920.2008.01580.x. 
      Epub 2008 Mar 12.