PMID- 18342010
OWN - NLM
STAT- MEDLINE
DCOM- 20080513
LR  - 20210103
IS  - 1097-4180 (Electronic)
IS  - 1074-7613 (Linking)
VI  - 28
IP  - 3
DP  - 2008 Mar
TI  - Restoring the association of the T cell receptor with CD8 reverses anergy in 
      human tumor-infiltrating lymphocytes.
PG  - 414-24
LID - 10.1016/j.immuni.2008.01.011 [doi]
AB  - For several days after antigenic stimulation, human cytolytic T lymphocyte (CTL) 
      clones exhibit a decrease in their effector activity and in their binding to 
      human leukocyte antigen (HLA)-peptide tetramers. We observed that, when in this 
      state, CTLs lose the colocalization of the T cell receptor (TCR) and CD8. 
      Effector function and TCR-CD8 colocalization were restored with galectin 
      disaccharide ligands, suggesting that the binding of TCR to galectin plays a role 
      in the distancing of TCR from CD8. These findings appear to be applicable in 
      vivo, as TCR was observed to be distant from CD8 on human tumor-infiltrating 
      lymphocytes, which were anergic. These lymphocytes recovered effector functions 
      and TCR-CD8 colocalization after ex vivo treatment with galectin disaccharide 
      ligands. The separation of TCR and CD8 molecules could be one major mechanism of 
      anergy in tumors and other chronic stimulation conditions.
FAU - Demotte, Nathalie
AU  - Demotte N
AD  - Ludwig Institute for Cancer Research, 1200 Brussels, Belgium; Cellular Genetics 
      Unit, Institute of Cellular Pathology, Universite catholique de Louvain, 1200 
      Brussels, Belgium.
FAU - Stroobant, Vincent
AU  - Stroobant V
FAU - Courtoy, Pierre J
AU  - Courtoy PJ
FAU - Van Der Smissen, Patrick
AU  - Van Der Smissen P
FAU - Colau, Didier
AU  - Colau D
FAU - Luescher, Immanuel F
AU  - Luescher IF
FAU - Hivroz, Claire
AU  - Hivroz C
FAU - Nicaise, Julie
AU  - Nicaise J
FAU - Squifflet, Jean-Luc
AU  - Squifflet JL
FAU - Mourad, Michel
AU  - Mourad M
FAU - Godelaine, Daniele
AU  - Godelaine D
FAU - Boon, Thierry
AU  - Boon T
FAU - van der Bruggen, Pierre
AU  - van der Bruggen P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (CD8 Antigens)
RN  - 0 (Galectins)
RN  - 0 (HLA Antigens)
RN  - 0 (Peptides)
RN  - 0 (Receptors, Antigen, T-Cell)
SB  - IM
MH  - CD8 Antigens/immunology/*metabolism
MH  - Cell Line, Tumor
MH  - Clonal Anergy/*immunology
MH  - Flow Cytometry
MH  - Galectins/metabolism
MH  - HLA Antigens/immunology
MH  - Humans
MH  - Lymphocyte Activation/immunology
MH  - Lymphocytes, Tumor-Infiltrating/immunology/*metabolism
MH  - Microscopy, Confocal
MH  - Microscopy, Electron, Scanning
MH  - Peptides/immunology
MH  - Receptors, Antigen, T-Cell/immunology/*metabolism
MH  - T-Lymphocytes, Cytotoxic/immunology/*metabolism
EDAT- 2008/03/18 09:00
MHDA- 2008/05/14 09:00
CRDT- 2008/03/18 09:00
PHST- 2007/07/12 00:00 [received]
PHST- 2007/12/18 00:00 [revised]
PHST- 2008/01/09 00:00 [accepted]
PHST- 2008/03/18 09:00 [pubmed]
PHST- 2008/05/14 09:00 [medline]
PHST- 2008/03/18 09:00 [entrez]
AID - S1074-7613(08)00070-8 [pii]
AID - 10.1016/j.immuni.2008.01.011 [doi]
PST - ppublish
SO  - Immunity. 2008 Mar;28(3):414-24. doi: 10.1016/j.immuni.2008.01.011.