PMID- 18342975
OWN - NLM
STAT- MEDLINE
DCOM- 20080716
LR  - 20211020
IS  - 1873-4995 (Electronic)
IS  - 0168-3659 (Linking)
VI  - 127
IP  - 3
DP  - 2008 May 8
TI  - Pulsatile release of biomolecules from polydimethylsiloxane (PDMS) chips with 
      hydrolytically degradable seals.
PG  - 280-7
LID - 10.1016/j.jconrel.2008.02.001 [doi]
AB  - We demonstrate, for the first time, a robust novel polydimethylsiloxane (PDMS) 
      chip that can provide controlled pulsatile release of DNA based molecules, 
      proteins and oligonucleotides without external stimuli or triggers. The PDMS chip 
      with arrays of wells was constructed by replica molding. Poly(lactic 
      acid-co-glycolic acid) (PLGA) polymer films of varying composition and thickness 
      were used as seals to the wells. The composition, molecular weight and thickness 
      of the PLGA films were all parameters used to control the degradation rate of the 
      seals and therefore the release profiles. Degradation of the films followed the 
      PLGA composition order of 50:50 PLGA>75:25 PLGA>85:15 PLGA at all time-points 
      beyond week 1. Scanning electron microscopy images showed that films were 
      initially smooth, became porous and ruptured as the osmotic pressure pushed the 
      degrading PLGA film outwards. Pulsatile release of DNA was controlled by the 
      composition and thickness of the PLGA used to seal the well. Transfection 
      experiments in a model Human Embryonic Kidney 293 (HEK293) cell line showed that 
      plasmid DNA loaded in the wells was functional after pulsatile release in 
      comparison to control plasmid DNA at all time-points. Thicker films degraded 
      faster than thinner films and could be used to fine-tune the release of DNA over 
      day length periods. Finally the PDMS chip was shown to provide repeated 
      sequential release of CpG oligonucleotides and a model antigen, Ovalbumin (OVA), 
      indicating significant potential for this device for vaccinations or applications 
      that require defined complex release patterns of a variety of chemicals, drugs 
      and biomolecules.
FAU - Intra, Janjira
AU  - Intra J
AD  - Division of Pharmaceutics, College of Pharmacy, University of Iowa, 52242, United 
      States.
FAU - Glasgow, Justin M
AU  - Glasgow JM
FAU - Mai, Hoang Q
AU  - Mai HQ
FAU - Salem, Aliasger K
AU  - Salem AK
LA  - eng
GR  - T32 GM007337/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080211
PL  - Netherlands
TA  - J Control Release
JT  - Journal of controlled release : official journal of the Controlled Release 
      Society
JID - 8607908
RN  - 0 (CpG ODN 1826)
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Silicones)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 63148-62-9 (baysilon)
RN  - 9006-59-1 (Ovalbumin)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Cell Line
MH  - DNA/administration & dosage/*chemistry
MH  - Dimethylpolysiloxanes/administration & dosage/*chemistry
MH  - Humans
MH  - Hydrolysis
MH  - Lactic Acid/administration & dosage/*chemistry
MH  - Oligodeoxyribonucleotides
MH  - Ovalbumin/administration & dosage/*chemistry
MH  - Plasmids/genetics
MH  - Polyglycolic Acid/administration & dosage/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Silicones/administration & dosage/*chemistry
MH  - Transfection
EDAT- 2008/03/18 09:00
MHDA- 2008/07/17 09:00
CRDT- 2008/03/18 09:00
PHST- 2007/12/06 00:00 [received]
PHST- 2008/01/28 00:00 [revised]
PHST- 2008/02/04 00:00 [accepted]
PHST- 2008/03/18 09:00 [pubmed]
PHST- 2008/07/17 09:00 [medline]
PHST- 2008/03/18 09:00 [entrez]
AID - S0168-3659(08)00076-X [pii]
AID - 10.1016/j.jconrel.2008.02.001 [doi]
PST - ppublish
SO  - J Control Release. 2008 May 8;127(3):280-7. doi: 10.1016/j.jconrel.2008.02.001. 
      Epub 2008 Feb 11.