PMID- 18343472
OWN - NLM
STAT- MEDLINE
DCOM- 20090107
LR  - 20080708
IS  - 0040-8166 (Print)
IS  - 0040-8166 (Linking)
VI  - 40
IP  - 4
DP  - 2008 Aug
TI  - Brain-derived neurotrophic factor (BDNF)-like immunoreactivity localization in 
      the retina and brain of Cichlasoma dimerus (Teleostei, Perciformes).
PG  - 261-70
LID - 10.1016/j.tice.2008.01.001 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) is a neurotrophin involved in the 
      development and maintenance of vertebrate nervous systems. Although there were 
      several studies in classical animal models, scarce information for fish was 
      available. The main purpose of this study was to analyze the distribution of BDNF 
      in the brain and retina of the cichlid fish Cichlasoma dimerus. By 
      immunohistochemistry we detected BDNF-like immunoreactive cells in the cytoplasm 
      and the nuclei of the ganglion cell layer and the inner nuclear layer of the 
      retina. In the optic tectum, BDNF-like immunoreactivity was detected in the 
      nucleus of neurons of the stratum periventriculare and the stratum marginale and 
      in neurons of the intermediate layers. In the hypothalamus we found BDNF-like 
      immunoreactivity mainly in the cytoplasm of the nucleus lateralis tuberis and the 
      nucleus of the lateral recess. To confirm the nuclear and cytoplasm localization 
      of BDNF we performed subcellular fractionation, followed by Western blot, 
      detecting a 39 kDa immunoreactive-band corresponding to a possible precursor form 
      of BDNF in both fractions. BDNF-like immunoreactivity was distributed in areas 
      related with photoreception (retina), the integration center of retinal 
      projections (optic tectum) and the control center of background and stress 
      adaptation (hypothalamus). These results provide baseline anatomical information 
      for future research about the role of neurotrophins in the adult fish central 
      nervous system.
FAU - Vissio, P G
AU  - Vissio PG
AD  - CONICET, Laboratorio de Embriologia Animal, DBBE, Facultad de Ciencias Exactas y 
      Naturales, Universidad de Buenos Aires, Ciudad Universitaria, C1428EHA Buenos 
      Aires, Argentina. paulav@bg.fcen.uba.ar
FAU - Canepa, M M
AU  - Canepa MM
FAU - Maggese, M C
AU  - Maggese MC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080317
PL  - Scotland
TA  - Tissue Cell
JT  - Tissue & cell
JID - 0214745
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Brain/cytology/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Fractionation
MH  - Hypothalamus/cytology
MH  - Immunohistochemistry
MH  - Neurons/cytology/metabolism
MH  - Perciformes/*metabolism
MH  - Protein Transport
MH  - Retina/cytology/*metabolism
MH  - Superior Colliculi/cytology/metabolism
EDAT- 2008/03/18 09:00
MHDA- 2009/01/08 09:00
CRDT- 2008/03/18 09:00
PHST- 2007/10/01 00:00 [received]
PHST- 2008/01/11 00:00 [revised]
PHST- 2008/01/14 00:00 [accepted]
PHST- 2008/03/18 09:00 [pubmed]
PHST- 2009/01/08 09:00 [medline]
PHST- 2008/03/18 09:00 [entrez]
AID - S0040-8166(08)00003-7 [pii]
AID - 10.1016/j.tice.2008.01.001 [doi]
PST - ppublish
SO  - Tissue Cell. 2008 Aug;40(4):261-70. doi: 10.1016/j.tice.2008.01.001. Epub 2008 
      Mar 17.