PMID- 18343554
OWN - NLM
STAT- MEDLINE
DCOM- 20080710
LR  - 20191210
IS  - 0278-6915 (Print)
IS  - 0278-6915 (Linking)
VI  - 46
IP  - 6
DP  - 2008 Jun
TI  - Comparison of the skin sensitizing potential of unsaturated compounds as assessed 
      by the murine local lymph node assay (LLNA) and the guinea pig maximization test 
      (GPMT).
PG  - 1896-904
LID - 10.1016/j.fct.2008.01.019 [doi]
AB  - The skin sensitization potential of eight unsaturated and one saturated lipid 
      (bio)chemicals was tested in both the LLNA and the GPMT to address the hypothesis 
      that chemicals with unsaturated carbon-carbon double bonds may result in a higher 
      number of unspecific (false positive) results in the LLNA compared to the GPMT. 
      Seven substances (oleic acid, linoleic acid, linolenic acid, undecylenic acid, 
      maleic acid, squalene and octinol) gave clear positive results in the LLNA 
      (stimulation index (SI)> or = 3) and thus would require labelling as skin 
      sensitizer. Fumaric acid and succinic acid gave clearly negative results. In the 
      GPMT, besides some sporadic skin reactions, reproducible skin reactions 
      indicating an allergic response were found in a few animals for four test 
      substances. Based on the GPMT results, only undecylenic acid would have to be 
      classified and labelled as a skin sensitizer according to the European Dangerous 
      Substance Directive (67/548/EEC) (results for linoleic acid were inconclusive), 
      while the other seven test substances would not require labelling. Possible 
      mechanisms for unspecific skin cell stimulation and lymph node responses are 
      discussed. In conclusion, the suitability of the LLNA for unsaturated compounds 
      bearing structural similarity to the tested substances should be carefully 
      considered and the GPMT should remain available as an accepted test method for 
      skin sensitization hazard identification.
FAU - Kreiling, R
AU  - Kreiling R
AD  - Clariant Produkte (Deutschland) GmbH, D-65840 Sulzbach, Germany.
FAU - Hollnagel, H M
AU  - Hollnagel HM
FAU - Hareng, L
AU  - Hareng L
FAU - Eigler, D
AU  - Eigler D
FAU - Lee, M S
AU  - Lee MS
FAU - Griem, P
AU  - Griem P
FAU - Dreessen, B
AU  - Dreessen B
FAU - Kleber, M
AU  - Kleber M
FAU - Albrecht, A
AU  - Albrecht A
FAU - Garcia, C
AU  - Garcia C
FAU - Wendel, A
AU  - Wendel A
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
DEP - 20080120
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (Fatty Acids, Unsaturated)
SB  - IM
MH  - Animals
MH  - Dermatitis, Allergic Contact/*pathology
MH  - False Positive Reactions
MH  - Fatty Acids, Unsaturated/*toxicity
MH  - Female
MH  - Guinea Pigs
MH  - *Local Lymph Node Assay
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Organ Size/drug effects
MH  - Reproducibility of Results
MH  - Structure-Activity Relationship
MH  - Toxicity Tests/*methods
EDAT- 2008/03/18 09:00
MHDA- 2008/07/11 09:00
CRDT- 2008/03/18 09:00
PHST- 2007/09/26 00:00 [received]
PHST- 2007/12/18 00:00 [revised]
PHST- 2008/01/12 00:00 [accepted]
PHST- 2008/03/18 09:00 [pubmed]
PHST- 2008/07/11 09:00 [medline]
PHST- 2008/03/18 09:00 [entrez]
AID - S0278-6915(08)00041-0 [pii]
AID - 10.1016/j.fct.2008.01.019 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 2008 Jun;46(6):1896-904. doi: 10.1016/j.fct.2008.01.019. Epub 
      2008 Jan 20.