PMID- 18344497
OWN - NLM
STAT- MEDLINE
DCOM- 20080612
LR  - 20190703
IS  - 0042-4900 (Print)
IS  - 0042-4900 (Linking)
VI  - 162
IP  - 11
DP  - 2008 Mar 15
TI  - Elimination of porcine reproductive and respiratory syndrome virus from a 
      seedstock breeding farm and a supplying boar stud by a modified test and removal 
      method.
PG  - 333-7
AB  - To eliminate porcine reproductive and respiratory syndrome virus (PRRSV) from a 
      supplying boar stud, samples of serum and semen from 118 boars were assessed 
      three times a month by an indirect fluorescent antibody (IFA) test to detect 
      antibodies, and by a nested reverse transcriptase-PCR (nRT-PCR) to detect the 
      genome of PRRSV. The boars detected as persistently infected carriers were 
      culled. A PRRSV-negative population of boars was established after three months 
      and no semen positive for the virus was detected for six months. Subsequently, a 
      three-step plan was introduced to eliminate PRRSV from the seedstock breeding 
      farm during three parity cycles on the farm over 15 months, each step taking five 
      months. In step 1, umbilical cords taken from piglets at birth and serum samples 
      taken from their dams at the start of weaning were subjected to ifa and nRT-pcr 
      analysis. The sows with PRRSV detected in serum by nRT-pcr were regarded as 
      carrier sows and culled. The rates of detection of PRRSV were reduced from 5 
      percent to 2.5 percent in the sera of the sows, and from 14.8 percent to 1.8 
      percent in the umbilical cords of the piglets. In step 2, the sows that had 
      farrowed the piglets with PRRSV detected by nRT-PCR in their cords were 
      considered to have transmitted the infection and removed. During step 2, the 
      virus detection rates in umbilical cords by nRT-pcr were reduced, but not 
      completely eliminated. In step 3, 10-week-old nursery pigs with antibodies to 
      PRRSV in their serum by ifa and elisa were culled. The three steps established 
      the PRRSV-negative state of the multisite farm containing the breeding and 
      nursery farm, and the PRRSV-negative state of both the multisite farm and the 
      supplying boar stud was evaluated by monthly monitoring over at least one parity 
      cycle of the farm for five months.
FAU - Yang, J S
AU  - Yang JS
AD  - Department of Veterinary Medicine Virology Laboratory, College of Veterinary 
      Medicine and BK21 Program for Veterinary Science, Seoul National University, 
      Seoul 151-742, Korea.
FAU - Moon, H J
AU  - Moon HJ
FAU - Lee, C S
AU  - Lee CS
FAU - Park, S J
AU  - Park SJ
FAU - Song, D S
AU  - Song DS
FAU - Kang, B K
AU  - Kang BK
FAU - Choi, J U
AU  - Choi JU
FAU - Park, B K
AU  - Park BK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Vet Rec
JT  - The Veterinary record
JID - 0031164
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - *Breeding
MH  - Carrier State/veterinary
MH  - Enzyme-Linked Immunosorbent Assay/methods/veterinary
MH  - Female
MH  - Fetal Blood/virology
MH  - Fluorescent Antibody Technique/methods/veterinary
MH  - Male
MH  - Parity
MH  - Porcine Reproductive and Respiratory Syndrome/*prevention & control/*transmission
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & 
      purification/metabolism
MH  - Pregnancy
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods/veterinary
MH  - Semen/virology
MH  - Swine
MH  - Time Factors
EDAT- 2008/03/18 09:00
MHDA- 2008/06/13 09:00
CRDT- 2008/03/18 09:00
PHST- 2008/03/18 09:00 [pubmed]
PHST- 2008/06/13 09:00 [medline]
PHST- 2008/03/18 09:00 [entrez]
AID - 162/11/333 [pii]
AID - 10.1136/vr.162.11.333 [doi]
PST - ppublish
SO  - Vet Rec. 2008 Mar 15;162(11):333-7. doi: 10.1136/vr.162.11.333.